聚合人脐带血红蛋白对复苏失血性休克大鼠肺组织的影响  

Effect of PolyCHb on pulmonary tissue of resuscitating hemorrhagic shock rats

作　　者：李红英 周文涛[1] 李遥金[1] 王京 李燊[1] 王红[1] 杨成民[1] 刘嘉馨[1] LI Hongying;ZHOU Wentao;LI Yaojin;WANG Jing;LI Shen;WANG Hong;YANG Chengmin;LIU Jiaxin(Institute of Blood Transfusion,Chinese Academy of Medical Sciences & Peking Union Medical College,Chengdu 610052,China)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2019年第2期132-136,共5页Chinese Journal of Blood Transfusion

基　　金：中国医学科学院医学与健康科技创新工程(2016-I2M-1-018;2017-I2M-3-021)

摘　　要：目的探讨不同浓度聚合人脐带血红蛋白(PolyCHb)复苏对失血性休克大鼠肺组织的影响。方法建立Wistar大鼠失血性休克控压模型:将40只健康雄性Wistar大鼠随机均分为假手术(sham)组、生理盐水(对照)组、2%、4%及6%PolyCHb组(8只/组)。sham组:大鼠麻醉后仅行股动、静脉插管;后4组建立大鼠失血性休克控压模型,分别给予生理盐水、生理盐水加相应浓度的PolyCHb复苏。分别在大鼠放血前(基础值)、休克、液体回输后0 h(复苏后0 h)、6 h(复苏后6 h)观测各组大鼠平均动脉压(MAP)、动脉二氧化碳分压(PaCO_2)、肺氧合指数(PaO_2/FiO_2);复苏后6 h以放血法处死大鼠,收集支气管肺泡灌洗液(BALF),测定蛋白含量,取肺组织,测定湿/干重比(W/D)值,另取肺组织制备成10%的组织匀浆,测定丙二醛(MDA)、髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)的含量。结果 1)复苏后0 h,2%、4%、6%PolyCHb组与对照组的MAP(mmHg)为117.25±5.97 vs 132.00±5.98 vs 147.75±5.82 vs 101.13±6.15(P<0.05)。2)复苏后6 h,4%、6%PolyCHb组与对照组相比,MAP(mmHg)分别为114.75±5.26 vs 118.63±13.81 vs 88.38±8.00(P<0.05);2%、4%PolyCHb组和对照组的PaCO_2(mmHg)为37.62±3.62 vs 37.13±4.70 vs 25.38±4.10(P<0.05);2%PolyCHb组和对照组的PaO_2/FiO_2值为463.69±44.74 vs 403.57±59.73,BALF蛋白浓度值(mg/mL)为0.13±0.04 vs 0.23±0.06(P<0.05), MDA(nmol/mg protein)为1.17±0.11 vs 1.47±0.20,MPO(U/g tissue)为0.37±0.08 vs 0.76±0.23(均为P<0.05);2%、4%、6%PolyCHb组和对照组的SOD(U/mg protein)为2.04±0.27 vs 1.87±0.30 vs 1.63±0.10 vs 1.83±0.04(均为P>0.05)。结论具有携氧功能的低浓度PolyCHb可以减轻失血性休克大鼠肺损伤;但随着PolyCHb浓度的升高,大鼠氧化应激增强,反而加重肺损伤。Objective To explore the effect of PolyCHb with different concentrations on pulmonary tissue of resuscitating hemorrhagic shock rats.Methods The hemorrhagic shock model was established, and 40 Wistar rats were randomly divided into one of the five groups(8 rats each group):sham group(femoral artery/vein intubation);0.9%NaCl(control) group(femoral artery/vein intubation and resuscitation with 0.9% NaCl solution);2%,4%, and 6% PolyCHb groups(femoral artery/vein intubation and resuscitation with corresponding concentration PolyCHb). The mean arterial pressure(MAP), carbon dioxide pressure(PaCO2) and pulmonary oxygenation index(PaO2/FiO2) were measured at the following four time-points:baseline immeditately before hemorrhage,,shock,immediately after resuscitation and 6 h after resuscitation, respectively.The rats were sacrificed by bloodletting at 6 h after resuscitation. The bronchoalveolar lavage fluid(BALF) was collected to determine the protein content;the wet-to-dry weight(W/D) ratio of lung tissue was measured. The lung tissue was excised to prapare 10% tissue homogenates, and the concentration of malondialdehyde(MDA), myeloperoxidase(MPO), and superoxide dismutase(SOD) of it were measured.Results 1) 0 h MAP(mmHg) of different concentration of PolyCHb(2%,4%,6%) groups and control group were 117.25±5.97 vs 132.00±5.98 vs 147.75±5.82 vs 101.13±6.15(P<0.05);2) 6 h MAP(mmHg) of 4%, 6%PolyCHb groups and control group were 114.75±5.26 vs 118.63±13.81 vs 88.38±8.00(P<0.05);the PaCO2(mmHg) of 2%, 4% PolyCHb groups and control group were 37.62±3.62 vs 37.13±4.70 vs 25.38±4.10(P<0.05);PaO2/FiO2 in 2% PolyCHb group and control group were 463.69±44.74 vs 403.57±59.73, and the protein content(mg/ml) of BALF were 0.13±0.04 vs 0.23±0.06(P<0.05);the concentration of MDA(nmol/mg protein) were 1.17±0.11 vs 1.47±0.20, and MPO(U/g tissue) were 0.37±0.08 vs 0.76±0.23(P<0.05);the levels of SOD(U/mg protein) in 2%,4%, 6% PolyCHb groups and control group were 2.04±0.27 vs 1.87±0.30 vs 1.63±0.10 vs 1.83±0.04(P>0.0

关 键 词：聚合人脐带血红蛋白 失血性休克 肺损伤 氧化应激 动物实验 大鼠 

分 类 号：R457.1[医药卫生—治疗学] R331.141[医药卫生—临床医学]