益智仁缩尿功效成分的研究  

Study on various components of Alpinia oxyphylla Fructus in urine-reducing effect

作　　者：冯惠敏 王周平[1] 徐德平[1] FENG Hui-min;WANG Zhou-ping;XU De-ping(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与机械》2019年第3期172-175,181,共5页Food and Machinery

基　　金：中央高校基本科研业务费专项资金(编号:JUSRP21127)

摘　　要：探究了益智仁的缩尿功效,并对其有效成分进行了分离鉴定。通过建立小鼠多尿模型,对有效部位进行缩尿活性筛选,采用代谢笼收集小鼠前2,6h内总尿量,并分别对其尿重/体重进行计算。结果表明,75%乙醇提取物乙酸乙酯萃取物对小鼠排尿的抑制效果最为明显,该部分经MCI、ODS-D柱分离,得到4种单体化合物,包括2个脂肪酸。运用NMR等波谱方法,并参考相关文献,对所得化合物进行结构分析,分别鉴定为3-甲氧基-4羟基-二苯己烷、20-丙基-β-谷甾醇、油酸、亚油酸。3-甲氧基-4羟基-二苯己烷和20-丙基-β-谷甾醇为首次从益智仁中分离得到并证实具有缩尿作用。This research focus on the efficiency of Alpinia Oxyphylla Fructus in urine-reducing and developing the method of separating and identifying its active components.Established a mice model with diuresis to validate the Alpinia Oxyphylla Fructus’ different components and purified by varying measures in reducing urine.The total urine volume in the first 2 and 6 hours of the mice was collected by metabolic cage.The values of urine weight/body weight were calculated respectively.The result showed that 75% ethanol extract of ethyl acetate phase was the most sensitive part in urine-reducing.Four pure monomer compounds were further separate from this part by MCI and ODS-D columns,including two fatty acids.The structure of components were identified as(1)3-methoxy-4-Hydroxy-diphenylhexane,(2)20-propyl-β-sitosterol,(3)oleic acid and(4)linoleic acid respectively,by the methods of NMR and other spectroscopy technology.Compound 1 and Compound 2 were separate from Alpinia Oxyphylla Fructus and confirmed to have effects on urine-reducing for the first time.

关 键 词：益智仁 缩尿 分离 鉴定 

分 类 号：R284[医药卫生—中药学] R285.5[医药卫生—中医学]