游泳池水引起的聚集性感染人腺病毒基因特征分析  

Genetic characteristics of adenovirus from clustered human cases occurring at a swimming pool

作　　者：杨鹏飞[1] 熊家声 燕清丽[1] 高强[1] 何芳 唐丽[1] 刘纯成[1] 赵怀荣[1] 姚海波[1] 熊成龙[3] 何南江 YANG Peng-fei;XIONG Jia-sheng;YAN Qing-li;GAO Qiang;HE Fang;TANG Li;LIU Chun-cheng;ZHAO Huai-rong;YAO Hai-bo;XIONG Cheng-long;HE Nan-jiang(Huai'an Municipal Center for Disease Control and Prevention,Huaian,Jiangsu ,China 223001;College of Maritie Science,Shandong University (Weihai;School of Public Health,Fudan University)

机构地区：[1]淮安市疾病预防控制中心,江苏淮安223001 [2]山东大学威海分校海洋学院 [3]复旦大学公共卫生学院

出　　处：《中国病原生物学杂志》2019年第4期420-425,共6页Journal of Pathogen Biology

基　　金：国家自然科学基金面上项目(No.81872673);淮安市自然科学研究计划项目(No.HAB201736);淮安市重点研发计划项目(No.HAS2015019-3);淮安市预防医学会科研基金项目(No.hayf201516)

摘　　要：目的了解淮安市一游泳池聚集性感染人腺病毒的hexon基因特征。方法采用实时荧光定量RT-PCR方法对12例游泳后出现呼吸道感染患儿咽拭子标本和2份游泳池水样进行呼吸道多病原体的快速筛查,对核酸阳性的标本进行hexon基因的部分序列扩增、测序并构建系统发生树,分析hexon基因特征及人腺病毒遗传多样性确定腺病毒的型别。结果 12例患儿中有9份咽拭子标本和1份儿童池游泳水样扩增出人腺病毒hexon基因片段,测序分析该毒株(淮安株)与我国GZ01株、日本J1007、美国株(USA27440、USA 5497、USA NHR3)核苷酸同源性及推导的氨基酸同源性均为99.9%。系统进化树分析显示,淮安株属于人腺病毒E亚属4基因型的进化谱系枝。结论该起聚集性呼吸道感染疫情可能由E亚属4基因型人腺病毒污染游泳池水引起。Objective To study the genetic characteristics of adenovirus from clustered human cases occurring at a municipal swimming pool.Methods Nasopharyngeal swabs were collected from 12 pediatric patients who developed an upper respiratory tract infection after swimming,and 2 samples of pool water were collected.Samples were screened for multiple respiratory pathogens using quantitative real-time RT-PCR.The genetic characteristics of and the phylogenetic relationship among human adenoviruses were determined by analyzing the sequence of the hexon gene.Results Human adenovirus nucleic acids were detected in 9 of 12 nasopharyngeal swabs and one sample of pool water.Comparison indicated that the Huai’an strains were very closely related to GZ01,J1007,USA27440,USA 5497,USA NHR3.Nucleotide sequences were 99.9%similar,and deduced amino acid sequences were 99.9%similar.A phylogenetic tree indicated the Huai’an strains clustered together and belonged to a genetic lineage of HAdV-4 within the E species.Conclusion Swimming pool water contaminated with HAdV-4 was the most likely source of infection.

关 键 词：聚集性病例 人腺病毒 hexon基因 进化分析 基因特征 

分 类 号：R373[医药卫生—病原生物学]