CRISPR/Cas9技术在人类重要病毒性传染病中的应用  

Use of the CRISPR/Cas9 technique in key human viral infectious diseases

作　　者：张珊[1] 胡康洪[1] 魏艳红[1] 孙鸽[1] 孙殿兴[2] ZHANG Shan;HU Kang-hong;WEI Yan hong;SUN Ge;SUN Dian-xing(Hubei University of Technology . Shio-Cremian Biomedical Center,Wuhan,China 430068;Department of Liner Diseases,Bethune International Peace Hospital,Chinese People's Liberation Army)

机构地区：[1]湖北工业大学生物医学中心工业微生物湖北省重点实验室,湖北武汉430068 [2]中国人民解放军白求恩国际和平医院肝病传染科

出　　处：《中国病原生物学杂志》2019年第4期491-496,F0003,共7页Journal of Pathogen Biology

基　　金：湖北省自然科学基金重点项目(No.2014CFA075)

摘　　要：近年来,CRISPR/Cas9作为一种新型的基因编辑工具,具备特异性强、使用广泛、操作相对简便等特点。通过对病毒基因组进行基因编辑从而干扰病毒复制和清除感染,以及在重要宿主因子、受体靶蛋白等方面应用都具有明显优势。本文着重以CRISPR/Cas9技术在一些导致人类重要传染性疾病的病毒如乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)、Epstein-Barr病毒(EBV)、单纯疱疹病毒(HSV)、寨卡病毒(ZIKV)、人类免疫缺陷病毒(HIV)中的应用、面临的技术瓶颈问题和解决方案及治疗前景进行讨论。Over the past few years, CRISPR/CAS9 has been developed into an innovative tool for gene editing. The advantages of CRISPR/CAS9 include a high degree of specificity, widely applicable use, and simplicity. The technology has obvious advantages in the following two aspects: 1) through gene editing of a viral genome, it can interfere with viral replication and eliminate infection and 2) it can be used to explore the interaction of viruses with important host factors and receptor target proteins. This review focuses on the recent use of the CRISPR/CAS9 system in various types of viruses that cause human infectious diseases such as hepatitis B virus(HBV), hepatitis C virus(HCV), Epstein-Barr virus(EBV), herpes simplex virus(HSV), Zika virus(ZIKV), and human immunodeficiency virus(HIV). This review also discusses several bottlenecks faced by the technology, corresponding solutions, and prospects for clinical treatment.

关 键 词：CRISPR/Cas9 乙型肝炎病毒(HBV) 丙型肝炎病毒(HCV) Epstein-Barr病毒(EBV) 单纯疱疹病毒(HSV) 寨卡病毒(ZIKV) 人类免疫缺陷病毒(HIV) 综述 

分 类 号：R373[医药卫生—病原生物学]