清肝凉血解毒方和凉血活血方对咪喹莫特诱导银屑病样小鼠行为学及神经肽Y表达影响  

Effects of Qinggan Liangxue Jiedu Decoction and Liangxue Huoxue Decoction on Behavior and Expression of Neuropeptide Y in Psoriatic Mice Induced by Imiquimod

作　　者：刘正荣[1,2,3] 王燕 赵京霞[2] 底婷婷[2] 李宁飞[1,2,3] 蒙玉娇 李雪 翟春艳[1,2] 刘宇 郭简宁[1,2,3] 郭肖瑶 张璐[2] 陈朝霞 李萍 LIU Zhengrong;WANG Yan;ZHAO Jingxia;DI Tingting;LI Ningfei;MENG Yujiao;LI Xue;ZHAI Chunyan;LIU Yu;GUO Jianning;GUO Xiaoyao;ZHANG Lu;CHEN Zhaoxia;LI Ping(Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China;Beijing Key Laboratory of Clinic and Basic Research with Traditional Chinese Medicine on Psoriasis,Beijing Institute of Traditional Chinese Medicine,Beijing 100010,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京市中医研究所银屑病中医临床基础研究北京市重点实验室,北京100010 [3]北京中医药大学,北京100029

出　　处：《辽宁中医药大学学报》2019年第5期33-37,F0003,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81573974)

摘　　要：目的:观察清肝凉血解毒方(QGLXJD)、凉血活血方(LXHX)对咪喹莫特诱导的银屑病样小鼠行为学及神经肽Y(NPY)表达的影响。方法:30只BALB/c雄性小鼠,剔除背部毛发,随机分为空白对照组(C)、模型组(M)、清肝凉血解毒方组(QG)、凉血活血方组(LX)和甲氨蝶呤组(MTX)。背部涂抹5%咪喹莫特乳膏(IMQ)诱导皮肤银屑病样模型。采用银屑病皮损面积和疾病严重程度(Psoriasis area and severity index,PASI)每日进行评分,分别运用旷场及高架十字迷宫评价各组小鼠行为学差异,HE染色后光镜下测量表皮厚度;免疫组化法检测皮损中T淋巴细胞表面标志CD3+及表皮中增殖细胞核抗原(PCNA)表达情况;采用实时PCR技术检测皮损中IL-23、IL-1β、IL-12 mRNA表达水平;Western blot法检测皮损中NPY蛋白表达情况。结果:与C组相比,M组表皮厚度、PCNA、CD3+T细胞、IL-1β、IL-12、IL-23 mRNA表达均增加(P<0.05);其余组与M组相比,表皮薄,PCNA、CD3+T细胞、IL-1β、IL-12、IL-23 mRNA表达水平均降低(P<0.05)。与C组比较,银屑病样小鼠焦虑水平高,以M组最为明显;QG组与M组比较,焦虑水平低,而LX组与QG组比较,焦虑水平高;NPY在M组中表达最多,在LX、QG、C组表达依次减少。结论:清肝凉血解毒方能改善银屑病样小鼠的焦虑行为,下调NPY的表达水平,从整体角度改善小鼠银屑病样皮损。Objectives:To observe the effects of Qinggan Liangxue Jiedu(QGLXJD)Decoction and Liangxue Huoxue(LXHX)Decoction on behavior and the expression of neuropeptide Y in psoriatic mice induced by imiquimod. Methods:30 male BALB/c mice were randomly divided into blank control group,model group,QGLXJD group(QG),LXHX group(LX)and MTX group. The skin psoriasis model was induced by applying 5% imiquimod cream(IMQ)on the back. The psoriasis skin lesion area and severity index(PASI)were used for daily scoring,and the behavioral differences of mice in each group were evaluated by using the open-field and elevated plus maze(EPM)respectively. The thickness of the epidermis was measured under the light microscope after HE staining. The expression of CD3^+ T cells and PCNA were detected by immunohistochemistry. Meanwhile,we used real-time PCR to detect the expression levels of IL-23,IL-1β,and IL-12 mRNA,Western blot to NPY protein in skin lesions. Results:Compared with group C,the epidermal thickness,PCNA,CD3^+ T cells,IL-1β,IL-12,and IL-23 mRNA expression in group M were all increased(P<0.05). Compared with the M group,the epidermis was thinner,and the expression levels of PCNA,CD3^+ T cells,IL-1β,IL-12,and IL-23 mRNA were all decreased(P<0.05). Compared with group C,the anxiety level of psoriatic mice was higher,which was most obvious in group M. The anxiety level was low in the QG group compared with the M group,while the anxiety level was higher in the LX group compared with the QG group. NPY was highest in group M,and decreased in group LX,QG and C. Conclusion:QGLXJD Decoction can improve the anxious behavior of psoriatic mice,down-regulate the expression level of NPY,and improve the psoriatic skin lesions of mice from the overall perspective.

关 键 词：银屑病 清肝凉血解毒方 NPY 行为学 旷场 高架十字迷宫 

分 类 号：R285.5[医药卫生—中药学]