Box-Behnken设计-响应面法优化通腑养髓方提取工艺研究  

Optimization of Extraction of Tongfu Yangsui Decoction by Box-Behnken Design-response Surface Method

作　　者：饶娆 乔欧 金文芳 周亚洁 孙云鹏[2] 王国凯[2] 汪世靖 韩永升 RAO Rao;QIAO Ou;JIN Wenfang;ZHOU Yajie;SUN Yunpeng;WANG Guokai;WANG Shijing;HAN Yongsheng(Affiliated Hospital of Institute of Neurology,Anhui University of Chinese Medcine,Hefei230061,Anhui,China;Anhui Unversity of Chinese Medicine,Hefei 230012,Anhui,China)

机构地区：[1]安徽中医药大学神经病学研究所附属医院,安徽合肥230061 [2]安徽中医药大学,安徽合肥230012

出　　处：《辽宁中医药大学学报》2019年第5期61-63,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：安徽省自然科学基金资助项目(1708085QH190);安徽省大学生创新创业资助项目(201710369166)

摘　　要：目的:优化通腑养髓方的提取工艺。方法:在单因素试验的基础上,考察提取时间、料液比、提取次数3个因素对芍药苷、盐酸小檗碱含量和共有峰总面积综合评分的影响,采用Box-Behnken设计-响应面分析法预测最佳提取工艺。结果:最佳提取工艺为2倍量水浸泡30 min,料液比1∶12,提取3次,每次1 h。验证试验表明与预测值相对误差为1.41%。结论:确定的提取工艺简便易行、稳定,可为进一步研究提供基础。Objectives:To optimize the extraction process of Tongfu Yangsui Decoction. Methods:On the basis of the single-factor test,the effects of three factors on paeoniflorin, berberine hydrochloride and total peak area were investigated. The Box-Behnken design-response surface analysis method was used to predict the optimal extraction process and the validate experiment was conducted. Results:The optimum extraction process as fellow: the herbs was soaked in water for 30 min, then extracted 2 times with 1 h per each time, solid-liquid ratio 1∶12 with water as a solvent. Conclusion:The extraction process was feasible and stable, which could provide a reference for further study.

关 键 词：通腑养髓方 Box-Behnken设计-响应面分析法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]