晚期氧化蛋白产物对妊娠滋养细胞生物学功能的影响  

Modulatory effect of advanced oxidation protein products on biological function of human first trimester trophoblast cells

作　　者：王硕石[1] 王英兰[1] 张竣 折瑞莲[1] 钟梅[2] Wang Shuoshi;Wang Yinglan;Zhang Jun;Zhe Ruilian;Zhong Mei(Department of Obstetrics and Gynecology, 2nd Clinical Medical College of Jinan University, Shenzhen People′s Hospital, Shenzhen 518002, P.R. China;The Department of Obstetrics and Gynecology, Southern Medical University, Nanfang Hospital, Guangzhou 510515, P.R. China;The Department of Family Planning, 2nd Clinical Medical College of Jinan University, Shenzhen People′s Hospital, Shenzhen 518002, China)

机构地区：[1]暨南大学第二临床医学院深圳市人民医院妇产科,518002 [2]南方医科大学南方医院妇产科,广州510515 [3]暨南大学第二临床医学院深圳市人民医院计划生育科,518002

出　　处：《中华临床医师杂志（电子版）》2019年第2期129-135,共7页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81671466);广东省自然科学基金项目(2015A030313003);广东省自然科学基金项目(2018A0303100021);深圳市卫生计生系统科研项目(201505003);深圳市科技创新委员会知识创新计划(JCYJ20150403101028171)

摘　　要：目的 观察晚期氧化蛋白产物(AOPP)对妊娠滋养细胞(HTR8/SVneo)功能的影响。方法 体外培养HTR8/SVneo 细胞株,用次氯酸(HClO)氧化鼠白蛋白(MSA)体外制备AOPPMSA,将HTR8/SVneo 滋养细胞与不同浓度(50 μg/ml 组、100 μg/ml 组或200 μg/ml 组)的AOPPMSA共同培养。四甲基偶氮唑蓝(MTT)比色法检测滋养细胞的增殖能力,以平均吸光度(A)值表示;侵袭小室检测滋养细胞侵袭能力,以穿膜细胞数表示;检测上清液中滋养细胞分泌的β-HCG水平评价滋养细胞分化能力;Hoechest33258 染色检测滋养细胞凋亡。采用单因素方差分析比较不同浓度AOPP 下,滋养细胞的增殖能力、侵袭能力、细胞分泌β-HCG、细胞凋亡数的差异。结果 与对照组比较,AOPP 浓度为50 μg/ml 时,对HTR8SVneo 滋养细胞增殖、侵袭、分化能力及凋亡影响不明显(P > 0.05);AOPP 浓度为100 μg/ml 和200 μg/ml 时,AOPP 明显抑制滋养细胞增殖、侵袭和分化能力,并促进滋养细胞凋亡(P < 0.05)。结论 AOPP 可以抑制滋养细胞的增殖、侵袭、分化能力及诱导滋养细胞凋亡。降低血浆中AOPP 水平可能有利于改善滋养细胞生物学功能。Objective To evaluate the effects of advanced oxidation protein products (AOPP) on the biological function of first-trimester trophoblast cell line (HTR8/SVneo). Methods The HTR8/SVneo was cultured in vitro. Mouse serum albumin (MAS) was oxidized by hypochloric acid (HOCl) to prepare AOPP-MAS in vitro. Based on variation of AOPP-MAS, the HTR8/SVneo was classified into 50ug/ml group, 100ug/ml group and 200ug/ml group. methyl thiazolyl tetrazolium (MTT) test was used to assess the cell proliferation, which was showed by the mean absorbance as A value. Transwell was used to measured the cell invasion, which was showed by the numbers of cells migration from filter membrane.β-HCG secretion was used to assessed the cell differentiation. The apoptosis of trophoblast cells was detected by Hoechest33258 staining. One-way ANOVA was used to compare the proliferation, invasive ability,β-HCG, and apoptosis of trophoblast cells at different concentrations of AOPP. Results Compared with the control group, 50ug/ mlAOPP group had no significant effect on the proliferation, invasion, differentiation and apoptosis of HTR8SVneo cells (P > 0.05). However, when AOPP concentration was 100 ug/ml or 200 ug/ml, AOPP significantly inhibited the proliferation, invasion and differentiation of trophoblast cells, at the same time, it increased the apoptosis of trophoblast cells (P < 0.05). Conclusions AOPP could modulate trophoblast proliferation, invasion and differentiation, in the mean time, AOPP also can induce apoptosis in trophoblast cells. Down regulation of plasma AOPP levels may help to improve biological functions in trophoblast cells.

关 键 词：晚期氧化蛋白产物 妊娠滋养细胞 增殖能力 侵袭能力 分化能力 

分 类 号：R714[医药卫生—妇产科学]