乌腺金丝桃与当归配伍对MIRI大鼠保护作用及其相关机制的实验研究  

Experimental Study on Protective Effect and Related Mechanisms of Hypericum Attenuatum Choisy and Angelica sinensis in MIRI Rats

作　　者：李冀[1] 李想[1] 王琳 曹明明[1] LI Ji;LI Xiang;WANG Lin;CAO Mingming(Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China;State Administration of Traditional Chinese Medicine on Taiwan, Hong Kong and Macao ChineseMedicine Exchange and Cooperation Center,Beijing 100026, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]国家中医药管理局对台港澳中医药交流合作中心,北京100026

出　　处：《辽宁中医杂志》2019年第4期849-852,I0007,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81874426);黑龙江省博士后基金项目(LBH-Z16189);黑龙江中医药大学校科研基金资助(2015BS02);黑龙江省普通本科高等学校青年创新人才培养计划(UPYSCT-2018226);国家中医药管理局名老中医工作室项目

摘　　要：目的:探讨乌腺金丝桃与当归配伍对大鼠心肌缺血再灌注损伤(MIRI)的保护作用、血清中GSH-Px活力及心肌细胞凋亡蛋白Bcl-2、Bax表达的影响。方法:采用乌腺金丝桃与当归单味药及配伍比例1:1、1:2、2:1各组分别作用于MIRI大鼠,观察各给药组对MIRI大鼠心肌病理形态影响,及血清中SOD、MDA含量、NO活力的影响,筛选出对MIRI大鼠心肌具有最佳保护作用的配伍组合。利用GSH-Px试剂盒测定验证抑制氧自由基程度,采用免疫组化法检测Bcl-2、Bax蛋白表达。结果:2:1组可明显改善模型大鼠心肌病理形态,明显减少心肌细胞凋亡,增加SOD活力及降低MDA含量明显,NO含量明显增加(P <0. 01);与模型组比较,2:1组可提高GSH-Px活力(P <0. 05);与模型组比较,2:1组大鼠心肌Bcl-2蛋白表达明显上调,Bax的蛋白表达明显下调(P <0. 01)。结论:2:1组对MIRI具有明显的保护作用,2:1组可提高GSH-Px活力,明显上调模型大鼠心肌Bcl-2蛋白表达,下调Bax的蛋白表达,说明其可通过抑制氧自由基生成以及细胞凋亡而发挥抗心肌缺血再灌注损伤的作用。Objective: To investigate the protective effects of Hypericum attenuatum Choisy and Angelica sinensis on myocardial ischemia-reperfusion injury,the activity of GSH-Px and the expression of Bcl-2 and Bax in cardiomyocytes. Methods: MIRI rats were treated with different concentrations of 1: 1,1: 2 and 2: 1. The effects of each group on the pathological changes of myocardium in MIRI rats and serum SOD,MDA and NO contents were observed. The MIRI rat myocardium which had the best combination of protection was screened. The GSH-Px kit was used to measure the degree of inhibition of oxygen free radicals and the expressions of Bcl-2 and Bax protein were detected by immunohistochemistry. Results: Compared with model group,the ratio of2: 1 group could significantly improve the pathological morphology of myocardium,significantly decrease the cardiomyocyte apoptosis,increase the activity of SOD and decrease the contents of MDA( P < 0. 01) and NO content increased significantly( P <0. 01). Compared with model group,the ratio of 2: 1 group increased GSH-Px activity in myocardium( P < 0. 05). The Bcl-2 protein expression in 2: 1 group was significantly up-regulated and the protein expression of Bax was significantly down-regulated( P < 0. 01). Conclusion: The 2: 1 group can significantly up-regulate the expression of Bcl-2 protein and down-regulate the protein expression of Bax,indicating that it can exert anti-myocardial ischemia-reperfusion injury by inhibiting the formation of oxygen free radicals and apoptosis.

关 键 词：MIRI 乌腺金丝桃 当归 GSH-PX BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]