分子标记辅助离体叶片接种鉴定甜瓜蔓枯病极端抗感单株  

In vitro leaf inoculation combing with molecular marker selection to identify extreme resistance and susceptibility of melon to gummy stem blight

作　　者：任琴琴 鲁秀梅 柯思佳 钱春桃[1] REN Qinqin;LU Xiumei;KE Sijia;QIAN Chuntao(State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing210095,Jiangsu,China)

机构地区：[1]作物遗传与种质创新国家重点试验室·南京农业大学园艺学院

出　　处：《中国瓜菜》2019年第5期13-16,共4页China Cucurbits And Vegetables

基　　金：江苏省农业重大新品种创制项目(PZCZ201718)

摘　　要：为了构建抗感基因池,快速准确筛选抗感单株,进行抗蔓枯病基因的精细定位,加速育种进程,采用分子标记辅助离体叶片接种鉴定,筛选极端抗感单株。研究结果表明,利用甜瓜F2群体的96株植株,接种5d后快速准确筛选到抗感单株分别为17株和15株,抗感植株的离体叶片接种鉴定与分子标记筛选的相关系数分别达0.85和0.68。In order to rapidly and accurately screen resistant plants, gene pools of resistant and susceptible were constructed to fine map resistance genes to gummy stem blight , aiming to accelerate the breeding process.In this study, in vitro leaf inoculation combing with molecular marker-assisted selection was performed to identify extreme resistant plants. Results have shown that 96 indivduals of melon F2 population were used to screen rapidly and accurately 17 resitant plants and 15 susceptible plants 5 days after inoculation. The resistance and susceptible correlation coefficients of in vitro leaf inoculation identification and molecular marker screening results were 0.85 and 0.68, respectively.

关 键 词：甜瓜 蔓枯病 分子标记 离体叶片 接种鉴定 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]