UPLC法测定香砂养胃丸中木香烃内酯及去氢木香内酯含量  

Determination of Costunolide and Dehydrocostuslactone in Xiangsha Yangwei Pills by UPLC

作　　者：程雅婷 王晓云 王学涛 王艳博 刘凯娜 杨新宇 石子琪[2] Cheng Yating;Wang Xiaoyun;Wang Xuetao;Wang Yanbo;Liu Kaina;Yang Xinyu;Shi Ziqi(Qinhuangdao Food and DrugInspection Center,Hebei Qinhuangdao 066004,China;Jiangsu Chinese Medicine Institute)

机构地区：[1]秦皇岛市食品药品检验中心,河北秦皇岛066000 [2]江苏省中医药研究院

出　　处：《中国药师》2019年第5期948-950,共3页China Pharmacist

摘　　要：目的:建立UPLC法测定香砂养胃丸中木香烃内酯和去氢木香内酯的含量,以控制制剂中君药木香的质量。方法:采用ACQUITY UPLC BEH C_(18)(50 mm×2. 1 mm,1. 7μm)色谱柱,以乙腈-0. 1%磷酸为流动相,流速为0. 2 ml·min^(-1),测定波长为225 nm,柱温为30℃。结果:木香烃内脂、去氢木香内脂分别在1. 62～402. 98μg·ml^(-1)(r=0. 999 9)和1. 66～828. 80μg·ml^(-1)(r=0. 999 9)范围内线性关系良好,精密度、稳定性、重复性RSD均低于2. 0%,平均加样回收率分别为96. 65%(RSD=2. 05%)、92. 93%(RSD=2. 55%)。结论:该方法准确、可靠,可用于香砂养胃丸中木香烃内酯及去氢木香内酯的含量测定。Objective:To establish a UPLC method to determine the contents of costunolide and dehydrocostuslactone in Xiangsha Yangwei pills for the quality control of radix aucklandiae,the main herb in the formula.Methods:An ACQUITY UPLC C18(50 mm×2.1 mm,1.7μm)chromatographic column was applied with acetonitrile and 0.1%phosphoric acid as the mobile phase.The flow rate was 0.2 ml·min^-1,the detection wavelength was set at 225 nm,and the column temperature was 30℃.Results:The linear relationships of the two components were very good within the range of 1.62-402.98μg·ml^-1(r=0.999 9)for costunolide and 1.66-828.80μg·ml^-1(r=0.999 9)for dehydrocostulactone.The RSDs of precision,stability and repeatability were all below 2.0%.The recovery was 96.65%(RSD=2.05%)and 92.93%(RSD=2.55%),respectively.Conclusion:The method is accurate and reliable,which can be used for the determination of costunolide and dehyrocostuslactone in Xiangsha Yangwei pills.

关 键 词：超高效液相色谱法 香砂养胃丸 木香烃内酯 去氢木香内酯 含量测定 

分 类 号：R927.2[医药卫生—药学]