不同猪种背部皮下脂肪中miR-1和miR-369的表达差异及其与胴体、肉质性状相关性分析  

Analysis of miR-1 and miR-369 differential expressions in subcutaneous adipose tissue of different pig breeds and the relevance of the expressions with carcass and meat quality traits

作　　者：陶璇[1] 顾以韧[1] 梁艳[1] 杨雪梅[1] 邓小松[2] 钟志君 杨跃奎[1] 曾凯[1] 陈晓晖[1] 王言 何志平[1] 龚建军[1] 雷云峰[1] 应三成[1] 王卫[3] 吕学斌[1] TAO Xuan;GU Yiren;LIANG Yan;YANG Xuemei;DENG Xiaosong;ZHONG Zhijun;YANG Yuekui;ZENG Kai;CHEN Xiaohui;WANG Yan;HE Zhiping;GONG Jianjun;LEI Yunfeng;YING Sancheng;WANG Wei;LYU Xuebin(Key Laboratory of Animal Breeding and Genetics of Sichuan Province,Sichuan Academy of Animal Sciences,Chengdu 610066,China;Sichuan Province Green Food Development Center,Chengdu 61004,China;Key Laboratory of Meat Processing of Sichuan Province,Chengdu 610106,China)

机构地区：[1]四川省畜牧科学研究院/动物遗传育种四川省重点实验室,四川成都610066 [2]四川省绿色食品发展中心,四川成都610041 [3]成都大学肉类加工四川省重点实验室,四川成都610106

出　　处：《畜牧与兽医》2019年第5期1-5,共5页Animal Husbandry & Veterinary Medicine

基　　金：四川省青年科技基金(2017JQ0011;2016JQ0041);肉类加工四川省重点实验室开放基金项目(18-R-06);四川省科技支撑计划(2016NYZ0042;2016NYZ0026;2016NZ0108);四川省重大科技专项(2017NZDZX0002);四川省财政运行专项(SASA2014CZYX001);"十二五"农村领域国家科技计划(2015BAD03B02-10);国家现代农业产业技术体系四川创新团队(SCCXTD-001;SCCXTD-005;SCCXTD-009);国家生猪产业技术体系-国家生猪育种群联合育种体系建立与持续选育(CARS-35)

摘　　要：为了研究miRNA表达量与猪胴体及肉质性状的相关性,采用荧光定量PCR技术(qRT-PCR)检测了引进猪种大约克夏猪和6个四川省地方猪种背部皮下脂肪中miR-1和miR-369的差异表达情况。结果:除miR-1与藏猪差异不显著外,2种miRNA在大约克夏猪背部皮下脂肪中的表达水平显著高于地方猪种(P<0.05);地方猪种中,miR-1和miR-369表达水平分别在藏猪和雅南猪中最高;相关性分析显示,miR-1和miR-369在背部皮下脂肪中表达水平均与瘦肉率、多不饱和脂肪酸含量呈极显著正相关(P<0.01),与单不饱和脂肪酸含量均呈显著负相关(P<0.05),miR-369表达水平还与平均背膘厚呈极显著负相关(P<0.01)。试验表明miR-1和miR-369可能对猪脂肪沉积和单不饱和脂肪酸生成起负调控作用,对多不饱和脂肪酸生成起正调控作用。The expression levels of miR-1 and miR-369 in the back subcutaneous adipose tissues of imported Yorkshire pigs and six indigenous pig breeds in Sichuan Province were measured using quantitative real-time polymerase chain reactions(qRT-PCR) to study the relationship between the expression levels and carcass traits and meat quality.The results showed that the expression levels of the two miRNAs in the Yorkshire pigs were significantly higher than those in the indigenous pigs(P<0.05),but there was no significant difference between the Yorkshire pigs and the Tibetan pigs in miR-1 expression.And the expression levels of miR-1 and miR-369 were the highest in the Tibetan pigs and the Yanan pigs respectively among the six indigenous pigs.Relevance analysis showed that the expression levels of miR-1 and miR-369 had a significantly positive correlation with the lean meat and PUFAs percentages(P<0.01) in the pigs,but a significantly negative correlation with the MUFAs percentage(P<0.05).Furthermore,there was a very significant negative correlation between the expression level of miR-369 and the average back fat thickness(P<0.01) in the pigs.The present study indicated that miR-1 and miR-369 might have negative regulation effects on adipopexis and MUFAs production,but positive regulation effects on PUFAs production.

关 键 词：猪 MIR-1 miR-369 QRT-PCR 背部皮下脂肪 

分 类 号：S828.8[农业科学—畜牧学]