血卟啉单甲醚脂质体的制备及其体外光触发释放的研究  

Study on Preparation of Hematoporphyrin Monomethyl Ether-loaded Liposomes and Its Phototriggering Release Performance in Vitro

作　　者：姜丹[1] 吴琳[1] 缪旭[1] JIANG Dan;WU Lin;MIAO Xu(Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001,China)

机构地区：[1]江苏大学附属医院,江苏镇江212001

出　　处：《抗感染药学》2019年第3期376-379,共4页Anti-infection Pharmacy

摘　　要：目的:研究血卟啉单甲醚脂质体的制备及其体外光触发释放性能,以及对细胞毒性的影响,评价其体外光动力抗肿瘤的效果。方法:采用逆向蒸发法制备负载光敏剂血卟啉单甲醚(HMME)的脂质体(Lipo-HMME),以实现HMME的光触发释放;并利用激光粒径仪等手段对Lipo-HMME进行表征,考察其体外光触发释放性能和脂质体对HMME暗毒性及细胞摄取的影响。结果:所制备的脂质体粒径分布均一,并具有明显的光触发释放性能,在光照及无光照条件下,1 h释放百分率分别为67.36%和21.3%;Lipo-HMME可有效降低HMME的暗毒性,提高其光动力治疗效果。结论:Lipo-HMME具有良好的光触发释放功能及其光动力治疗效果,可作为载体使用于肿瘤患者的特异性治疗。Objective:To study the preparation and the effects on cytotoxicity of hematoporphyrin monomethyl ether(HMME) liposomes(Lipo-HMME)and its phototriggered release in vitro, and to evaluate the photodynamic antitumor effect in vitro. Methods: The Lipo-HMME loaded photosensitizer is prepared by reverse evaporation method.In addition,lipo-HMME is characterized by laser particle size analyzer and other means to investigate its in vitro light-triggered release performance and the effect of liposomes on dark toxicity and cell uptake of HMME. Results: The prepared liposomes have uniform particle size distribution and obvious light-triggered release performance. The 1 h release rates ware67.36% and 21.3%, respectively, under light and non-light conditions. Lipo-HMME can effectively reduce the dark toxicity of HMME and improve its photodynamic therapy effect. Conclusion: Lipo-HMME has good light-triggered release function and photodynamic therapeutic effect, which can be used as a carrier for specific treatment of tumor patients.

关 键 词：光动力治疗 血卟啉单甲醚 脂质体 光触发释放性能 

分 类 号：R944[医药卫生—药剂学]