有性三倍体杂交兰‘黄荷兰’的快速繁殖  

Micropropagation of Sexual Triploid Hybrid Cymbidium ‘Huanghe’

作　　者：徐仕英 李映雪 吴忧 朱娇 郭和蓉 曾瑞珍[1,2] XU Shiying;LI Yingxue;WU You;ZHU Jiao;GUO Herong;ZENG Ruizhen(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642;Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou,Guangdong 510642)

机构地区：[1]华南农业大学林学与风景园林学院,广东广州510642 [2]华南农业大学广东省植物分子育种重点实验室,广东广州510642

出　　处：《北方园艺》2019年第9期69-76,共8页Northern Horticulture

基　　金：广东省科技计划资助项目(2015A020209101;2017B020201010);广州市科技计划项目产学研协同创新重大专项资助项目(201604020065);广州市农业局资助项目(1610519;1710212);清远市科技计划资助项目(2017A005)

摘　　要：以‘黄荷兰’试管苗为试材,采用组织培养快速繁殖方法,研究了影响有性三倍体杂交兰‘黄荷兰’根状茎诱导、增殖、分化、生根壮苗和试管苗移栽的因素,以期建立‘黄荷兰’种苗工厂化生产技术体系。结果表明:横切茎尖的起始脱分化时间早于纵切,诱导率高于纵切。切割长度、外源激素、光照强度和有机附加物对根状茎的增殖影响显著,将根状茎切割成长0.50 cm接种到MS+6-BA 1.00 mg·L^(-1)+NAA 0.20 mg·L^(-1)+土豆80.00 g·L^(-1)+蔗糖30.00 g·L^(-1)+卡拉粉7.00 g·L^(-1)+AC 0.30 g·L^(-1)培养基中培养40 d,根状茎增殖系数为3.18。外源激素对根状茎的分化影响显著,将根状茎掰成长1.00 cm接入MS+6-BA 1.00 mg·L^(-1)+NAA 0.20 mg·L^(-1)+蔗糖20.00 g·L^(-1)+卡拉粉7.00 g·L^(-1)+AC 0.02 g·L^(-1)培养基中培养40 d,芽分化率和苗分化率分别为60.00%和57.50%。将4 cm高的苗转入1/2MS+6-BA 0.10 mg·L^(-1)+NAA 0.50 mg·L^(-1)+蔗糖20.00 g·L^(-1)+卡拉粉7.00 g·L^(-1)+AC 0.50 g·L^(-1)中培养50 d,平均株高和根数分别为7.32 cm和3.56条。4月和10月用水草移栽,试管苗成活率分别为98.67%和99.67%。上述研究结果说明,利用组织培养快速繁殖技术工厂化生产‘黄荷兰’种苗是可行的。In order to establish the technical system of micropropagation of sexual triploid hybrid Cymbidium ‘Huanghe’, the test-tube seedlings were employed to investigate the factors influencing on induction,proliferation and differentiation of rhizome,seedling strengthening and transplantation by using of tissue culture rapid propagation method. The results showed that the initial dedifferentiation time of shoot tip with transverse cutting was earlier and the induction rate was higher than that with longitudinal cutting. The length of rhizome, combination of exogenous hormone, light intensity and organic additives had significant effects on rhizome proliferation, and when rhizomes with 0. 50 cm in length were inoculated on MS+6-BA 1. 00 mg·L^-1 + NAA 0. 20 mg·L^-1 + potato 80. 00 g·L^-1 + sucrose 30. 00 g·L^-1 + AC 0. 30 g·L^-1 and cultured at 26 ℃ under 1 000 lx light intensity for 40 days, the proliferation coefficient was the highest (3.18). Combination of exogenous hormone significantly affected rhizome differentiation, and when rhizomes in 1.00 cm length were inoculated on MS+6-BA 1. 00 mg·L^-1 +NAA 0. 20 mg·L^-1 + sucrose 20. 00 g·L^-1 +AC 0. 02 g·L^-1 and cultured at 26 ℃ under 2 000 lx light intensity for 40 days, the bud and seedling differentiation rates were 60. 00% and 57. 50%, respectively. When seedlings with 4. 0 cm high were inoculated on 1/2MS+ 6- BA 0. 10 mg·L^-1 +NAA 0. 50 mg·L^-1 + sucrose 20. 00 g·L^-1 + AC 0. 50 g·L^-1 and cultured at 26 ℃ under 2 000 lx light intensity for 50 days,the average seedling height and mean number of root were 7.32 cm and 3.56, respectively. The transplantation survival rate of seedlings in April and October was 98.67% and 99.67 %, respectively by using of moss as culture substance. The above results demonstrate that it was feasible to produce seedlings of Cymbidium ‘Huanghe’ by the use of tissue culture rapid propagation technology.

关 键 词：杂交兰 有性多倍体 根状茎 快速繁殖 

分 类 号：S682.31[农业科学—观赏园艺]