生化和ISSR分子标记在黑木耳品种鉴定中的应用  

Application of Biochemical and ISSR Molecular Markers in Identification of Auricularia auricular

作　　者：史灵燕 刘保卫 顾新颖 郭金英 郑素月 张庆桥 SHI Lingyan;LIU Baowci;GU Xinying;GUO Jinying;ZHENG Suyue;ZHANG Qingqiao(College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan,Hebei 056021;Agricultural Bureau of Fuping,Baoding,Hebei 073200)

机构地区：[1]河北工程大学园林与生态工程学院,河北邯郸056021 [2]阜平县农业局,河北保定073200

出　　处：《北方园艺》2019年第9期136-141,共6页Northern Horticulture

基　　金：河北省现代农业产业技术体系食用菌创新团队建设专项资金资助项目(HBCT2018050202);阜平县太行山食用菌研究院资助项目(20170192)

摘　　要：以生产收集的24个黑木耳栽培菌株为试材,采用拮抗、酯酶同工酶及ISSR分子标记技术对其进行分类鉴定和遗传多样性分析。结果表明:酯酶同工酶酶谱显示24个黑木耳菌株共扩增出11条迁移率不同的酶带,聚类分析表明当遗传系数为0.74时,将24个黑木耳菌株分为两大类;通过ISSR分子标记共扩增出67条清晰的DNA多态片段,大小介于0.2～2.0 kb,聚类分析表明当遗传系数为0.85时,将24个菌株分为两大类,聚类分析结果与拮抗试验结果基本一致。该研究为黑木耳的菌株选择及遗传育种提供了参考依据。The antagonistic test,esterase isozyme and ISSR molecular marker technique were performed to analyze the genetic diversity of twenty-four cultivated strains of Auricularia auricula.The results showed that eleven distinct bands were detected by the esterase isozyme.Cluster analysis results showed that the twenty-four cultivated strains of Auricularia auricula were divided into two groups at genetic similarity of 0.74.Sixty-seven distinct bands which ranged from 0.2 kb to 2.0 kb were detected by gel electrophoresis.Cluster analysis results showed the twenty-four cultivated strains of Auricularia auricula were divided into two groups at genetic similarity of 0.85.The results of cluster analysis was similar with the antagonistic test.This study provided a scientific basis for strain selection in production and genetics breeding of Auricularia auricula.

关 键 词：黑木耳 拮抗 酯酶同工酶 ISSR 

分 类 号：S646.601[农业科学—蔬菜学]