外源水杨酸对草地早熟禾抗褐斑病的诱导与抗病基因PR1和NPR1的表达的影响  

Effects of exogenous salicylic acid on the resistance of Kentucky bluegrass to brown patch and expression of PR1 and NPR1 resistance genes

作　　者：尉春雪 苏浩天 张晓宇 何文菡 郑大柽 尹淑霞[1] WEI Chunxue;SU Haotian;ZHANG Xiaoyu;HE Wenhan;ZHENG Dacheng;YIN Shuxia(Forestry College of Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,北京100083

出　　处：《草业科学》2019年第5期1249-1254,共6页Pratacultural Science

基　　金：国家自然科学基金"空间诱变草地早熟禾矮化突变体矮化机理研究(31302016)"

摘　　要：为了探究外源水杨酸(SA)对草地早熟禾(Poa pratensis)抗褐斑病的诱抗效果,本研究将草地早熟禾品种午夜(Midnight)分为3组,用0.05 mmol·L^(–1) SA和立枯丝核菌进行处理,测定草坪草发病率、病情指数,计算诱抗效果,同时检测相关抗病基因PR1、NPR1表达情况。结果表明,外源SA可以显著(P <0.05)降低草地早熟禾褐斑病的发病率和病情指数,对褐斑病的诱抗效果最高达53%。在喷施SA及接种后,抗病基因PR1和NPR1相对表达量均显著(P <0.05)升高。To explore the effect of external salicylic acid(SA)on the resistance of Kentucky bluegrass(Poa pratensis)to brown patch,Kentucky bluegrass variety‘Midnight’was divided into three groups and treated with 0.05 mmol·L^–1 SA and Rhizoctonia solani.The percent of disease infection and disease index were measured,and the efficacy of resistance induced was calculated.The relative expression of disease resistance genes PR1 and NPR1 was assessed.The results showed that external SA could significantly(P<0.05)decrease the plant infection percent and disease index of brown patch on Kentucky bluegrass,and the highest efficacy of resistance induced by SA was 45.53% .After spraying SA and inoculation,the relative expression of PR1 and NPR1 increased significantly(P<0.05).

关 键 词：外源SA 草地早熟禾 褐斑病 发病率 病情指数 诱抗效果 抗病基因PR1和NPR1 

分 类 号：S688.4[农业科学—观赏园艺]