长穗偃麦草成熟种胚高频再生体系  

High-frequency regeneration system of mature embryos of Elytrigia elongata

作　　者：周妍彤 郭强[1] 毛培春[1] 田小霞[1] 崔国文[2] 孟林[1] ZHOU Yantong;GUO Qiang;MAO Peichun;TIAN Xiaoxia;CUI Guowen;MENG Lin(Beijing Research and Development Center for Grass and Environment,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]北京市农林科学院北京草业与环境研究发展中心,北京100097 [2]东北农业大学动物科技学院,黑龙江哈尔滨150030

出　　处：《草业科学》2019年第5期1317-1322,共6页Pratacultural Science

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20170110);北京市自然科学基金项目(6182013)

摘　　要：以长穗偃麦草(Elytrigia elongata)成熟胚为外植体,在MS基本培养基的基础上,附加不同浓度的2,4-D、6-BA和NAA等植物生长调节剂,开展对其愈伤组织诱导、绿苗分化和生根等的试验研究。结果表明,适宜愈伤组织诱导的培养基为MC (MS+30 g·L^(–1)麦芽糖+1 g·L^(–1) CH+200×5 mL·L^(–1) VB+0.5 g·L^(–1) L-Pro+3 g·L^(–1)植物凝胶)+3 mg·L–12,4-D+0.025 mg·L^(–1) 6-BA,诱导率达77.78%,4周后可见淡黄色愈伤组织;最佳分化培养基为MC (MS+30 g·L^(–1)麦芽糖+1 g·L^(–1) CH+200×5 mL·L^(–1) VB+0.5 g·L^(–1) L-Pro+3 g·L^(–1)植物凝胶)+0.1 mg·L^(–1) 2,4-D+3 mg·L^(–1) 6-BA,分化率达66.67%,4周后出现芽点,同时伴随根毛发生;最佳生根培养基为MR(1/2MS+15 g·L^(–1)麦芽糖+3 g·L^(–1)植物凝胶)+0.5 mg·L^(–1) NAA,移栽后全部成活。从而建立了一套从长穗偃麦草"成熟种胚–诱导愈伤组织–绿苗分化–生根–移栽"的组培再生体系,为进一步研究其抗逆分子机制奠定了重要基础。The mature embryos of Elytrigia elongata seeds were used as explants and MS was used as the basic medium to carry out research on callus induction,green plantlet differentiation,and rooting by adding different growth regulator media.The results showed that the suitable medium for callus induction was MC(MS+30 g·L^–1 Maltose+1 g·L^–1 CH+5 mL·L^–1 200×VB+0.5 g·L^–1 L-Pro+3 g·L^–1 Phytagel)+3 mg·L^–1 2,4-D+0.025 mg·L^–1 6-BA.The induction rate was 77.78% ,and the callus was visible after 4 weeks.The best differentiation medium was MC(MS+30 g·L^–1 Maltose+1 g·L^–1 CH+5 mL·L^–1 200×VB+0.5 g·L^–1 L-Pro+3 g·L^–1 Phytagel)+0.1 mg·L^–1 2,4-D+3 mg·L^–1 6-BA,and the differentiation rate was 66.67% .The budding point appeared after 4 weeks,accompanied by root hair development.The best rooting medium was MR(1/2 MS+15 g·L^–1 Maltose+3 g·L^–1 Phytagel)+0.5 mg·L^–1 NAA.All plantlets survived after transplanting.Therefore,differentiation tissue culture regeneration system for E.elongata was established,including mature embryosinducing callus-green plantlet differentiation-rooting-transplantation,which lays an important foundation for further study of its molecular mechanism of resilience.

关 键 词：MS 2 4-D 6-BA NAA 植物生长调节剂 组织培养 植株再生 

分 类 号：S512.934[农业科学—作物学]