山东栒子基因组DNA的提取和SRAP分子标记引物筛选  

Extraction of Genomic DNA from Cotoneaster schantungensis and Screening of SRAP Molecular Marker Primers

作　　者：步俊彦 臧德奎[1] BU Jun-yan;ZANG De-kui(College of Forestry,Shandong Agricultural University,Taian,Shandong 271000)

机构地区：[1]山东农业大学林学院

出　　处：《安徽农业科学》2019年第10期97-100,共4页Journal of Anhui Agricultural Sciences

基　　金：山东省科技创新项目(LYCX01-2018-03);国家自然科学基金项目(31870688)

摘　　要：根据山东栒子叶片多糖多酚多毛的理化性质,研究适合山东栒子便捷高效的基因组DNA提取方法。结果获得了纯度高、质量符合后续分子标记和遗传多样性分析要求的基因组DNA。采用6个不同种群的样品,利用150对SRAP引物组合进行筛选,确定了最佳反应体系为2×EsTaqMasterMix(含染料)12.5μL,40ng/μLDNA模板1μL,10pmol/μL引物0.5μL,ddH2O10.5μL,共筛选出11对多态性相对良好、条带较为清晰的引物组合。该研究为以后SRAP分子标记在栒子属植物遗传多样性方面的研究奠定了基础。According to the physicochemical properties of polysaccharide polyphenols and hypertrichiasis from Cotoneaster schantungensis, a convenient and efficient genomic DNA extraction method suitable for C.schantungensis was studied.The obtained genomic DNA was of high purity and the quality was sufficient to satisfy the requirements of subsequent molecular marker experiments.For 6 plant samples from different populations,150 pairs of SRAP primer combinations were used to screen,and 2×Es Taq MasterMix (containing dye) 12.5 μL,40 ng/μL DNA template 1 μL,10 pmol/μL primer 0.5 μL,dd H 2O 10 .5 μL of good system and 11 pairs of well-characterized bands with clear primer combinations were determined.This series of experiments laid the foundation for the experimental study of genetic diversity of SRAP molecular markers for the C.schantungensis and Cotoneaster genus species.

关 键 词：DNA提取 山东栒子 SRAP 引物筛选 

分 类 号：S188.1[农业科学—农业基础科学]