高效液相色谱法测定双葛止泻口服液中绿原酸的含量  

Determination of Chlorogenic Acid in Shuangge Anti-diarrhea Oral Liquid by HPLC

作　　者：周冰 李焕娟 杨会鲜 张聪 周德刚 ZHOU Bing;LI Huan-juan;YANG Hui-xian(National Research Center for Veterinary Medicine,Luoyang HuizhongVeterinary Medicine Co. ,Ltd.,Luoyang,Henan 471000)

机构地区：[1]国家兽用药品工程技术研究中心洛阳惠中兽药有限公司

出　　处：《安徽农业科学》2019年第10期167-169,共3页Journal of Anhui Agricultural Sciences

基　　金：2019年度洛阳市科技重大专项项目(1901024A)

摘　　要：[目的]建立并验证双葛止泻口服液中绿原酸的含量测定方法。[方法]采用AgilentZORBAXSB-C18(150mm×4.6mm,5μm),流动相为乙腈-0.4%磷酸水溶液梯度洗脱;检测波长为327nm;流速为1.0mL/min;柱温为25℃。[结果]绿原酸进样量在0.0825~0.8250μg与峰面积呈良好的线性关系(r=0.9999),平均回收率为104.0%,RSD为0.4%。[结论]该方法简便、准确、专属性强,可用于双葛止泻口服液中绿原酸的质量控制。[Objective] The method for determination of the content of chlorogenic acid in Shuangge anti-diarrhea oral liquid was developed and validated.[Method] The analysis was performed on an Agilent ZORBAX SB-C18 column(150 mm×4.6 mm, 5 μm). The gradient mobile phase was consisted of acetonitrile-0.4% phosphoric acid solution, and the detection wavelength was 327 nm. The flow rate was 1.0 mL/min and the column temperature was 25 ℃.[Result] The linear range of the calibration curves for chlorogenic acid was 0.082 5-0.825 0 μg(r = 0.999 9), and the average recovery was 104.0% with RSD of 0.4%.[Conclusion] The method is simple and accurate with high specificity, which can be used to determine the content of chlorogenic acid in Shuangge Anti-diarrhea oral liquid.

关 键 词：绿原酸 双葛止泻口服液 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]