免疫球蛋白IgY对牙龈卟啉单胞菌诱导的牙周炎大鼠模型的影响  

Effect of egg yolk immunoglobulin on an experimental periodontitis model induced by Porphyromonas gingivalis

作　　者：王芳 许啸晨[3] 吴文惠 WANG Fang;XU Xiao-chen;WU Wen-hui(College of Fundamental Medicine,Shanghai University of Medicine & Health Sciences,Shanghai 201318,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai MAXAM Company Limited,Shanghai 200333,China;Institute of Marine Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海健康医学院基础医学院,上海201318 [2]上海海洋大学食品学院,上海201306 [3]上海美加净日化有限公司,上海200333 [4]上海海洋大学海洋科学研究院,上海201306

出　　处：《华东师范大学学报（自然科学版）》2019年第3期131-137,共7页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(81502955,81750110548);上海市科技创新行动计划(17490742500)

摘　　要：研究了免疫球蛋白(IgY)通过抑制P.gingivalis的生长繁殖治疗实验性大鼠牙周炎的可行性.用医用丝线结扎上颌第二磨牙,并涂抹P.gingivalis的方法建立大鼠牙周炎模型.建模成功的大鼠随机分成对照组、高剂量IgY组和低剂量IgY组,每组8只.给药28 d后,测量各组大鼠的牙龈指数(GI),菌斑指数(PI)和探诊出血指数(BOP),以及血液中IL-6和TNF-α含量,并进行X-射线影像和组织学观察.与对照组相比,IgY治疗组GI、PI和BOP指标明显好转,IL-6和TNF-α含量显著降低,X-射线影像可见牙间隙明显缩小,组织学观察牙周纤维排列整齐,极少量炎细胞浸润,牙槽嵴无吸收,牙槽骨骨质增加.特异性IgY能够治疗P.gingivalis诱导的大鼠牙周炎.This study evaluates the therapeutic effects of egg yolk Immunoglobulin(IgY)on periodontitis in an established rat periodontitis model through the growth inhibition of Porphyromonas gingivalis.Experimental periodontitis was induced in mice by ligation of their second molar with medical thread and oral infection with Porphyromonas gingivalis.Periodontitis rats were randomly divided into control,high-dose,and low-dose groups.Each group was comprised of eight rats.The gingival index(GI),plaque index(PI),and bleeding on probing(BOP)were evaluated,the levels of IL-6 and TNF-αwere examined,and an X-ray image and histomorphological section were observed after 28 days.Compared with the control,the scores for PI,GI,and BOP and the levels of IL-6 and TNF-αwere significantly decreased in the IgY-treated groups.X-ray images showed that the tooth gap narrowed considerably.Pathological observation,furthermore,showed that periodontal fibers were neatly arranged,a small amount of inflammatory cells were infiltrated,the alveolar ridge was not absorbed,and alveolar bone increased.In conclusion,specific IgY can treat periodontitis induced by P.gingivalis in an established rat periodontitis model.

关 键 词：免疫球蛋白 IGY 牙周炎动物模型 牙周指数 IL-6 TNF-α X射线影像 病理组织切片 

分 类 号：Q939.91[生物学—微生物学]