应用磁共振体素内不相干运动成像评价大鼠肝纤维化  

Evaluation of liver fibrosis in rats by magnetic resonance intravoxel incoherence imaging

作　　者：王琳萍 裴彩侠 刘钊[2] 刘海峰[2] 张鹏飞[1] 田瑜 郑雅兰 王少彧 雷军强[2] WANG Linping;PEI Caixia;LIU Zhao;LIU Haifeng;ZHANG Pengfei;TIAN Yu;ZHENG Yalan;WANG Shaoyu;LEI Junqiang(The First School of Clinical Medicine, Lanzhou University, Lanzhou 730030, China;Department of Radiology, First Hospital of Lanzhou University, Gansu InternationalCooperation Base of Precision Image Collaborative Innovation, Lanzhou 730030, China;Magnetic Resonance Division Simens Medical Systems LTD Health Industry in GansuProvince, Shanghai 200000, China)

机构地区：[1]兰州大学第一临床医学院,兰州730030 [2]兰州大学第一医院放射科精准影像协同创新甘肃省国际合作基地,兰州730030 [3]西门子医疗系统有限公司磁共振事业部,上海200000

出　　处：《磁共振成像》2019年第5期371-376,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：甘肃省卫生行业科研计划基金项目(编号:GSWSKY2016-26);兰州大学第一临床医学院卓越计划基金项目(编号:20180060056;20180060060)~~

摘　　要：目的探讨磁共振体素内不相干运动(intravoxel incoherent motion,IVIM)评价大鼠肝纤维化的诊断及分期价值。材料与方法选取SPF级SD大鼠随机分实验组55只和对照组11只,实验组注射CCL4建立肝纤维化模型。第2～12周任意取实验组5只与对照组1只行MR扫描,测量扩散加权成像(diffusion weighted imaging,DWI)、IVIM参数值后同期行METAVIR分期病理检查。用SPSS 22.0分析DWI、IVIM参数值与肝纤维化分期的相关性、诊断及分期价值。结果 (1)实验组在建模期间4只死亡,6只扫描图像质量不佳被排除,共纳入45只实验鼠病理结果:F1～4分别为8、13、14、10只,对照组11只为F0。(2)实验组较对照组ADC、D*、f明显降低且差异具有统计学意义(P<0.01)。ADC、D、f、D*值随肝纤维化进展下降,且Spearman分析示ADC、D、f和D*与肝纤维化水平呈负相关(P<0.05)。(3) F3和F4的ADC、F0和F1的D*、F1和F2的D*和f、F3和F4的D差异有统计学意义(P<0.05)。受试者工作特征(receiver operating characteristic,ROC)曲线示D*鉴别F0和F1～F4、F0～F1和F2～F4及F0～F2和F3～F4效能优于ADC、D和f;ADC鉴别F0～F3和F4效能最高。结论 D*鉴别F0～F3效能最高,ADC鉴别F0～F3和F4效能最高。D*、ADC具肝纤维化分期应用价值。Objective: To evaluate the diagnostic value of intravoxel incoherent motion (IVIM) in the evaluation of liver fibrosis in rats. Materials and Methods: SPF SD rats were randomly divided into experimental group (55) and control group (11). The experimental group was established with hepatic fibrosis model. From the 2nd to the 12th week, 5 rats in the experimental group and 1 in the control group were randomly selected for MR scanning, and the METAVIR stage for diagnosis and diffusion weighted imaging (DWI) and IVIM parameter values were measured during the same period. SPSS 22.0 was used to analyze the correlation, diagnosis, and staging value of DWI and IVIM parameters with liver fibrosis stages. Results:(1) Four deaths and poor quality of 6 scanned images were excluded during modeling in the experimental group, and 45 experimental mice were eventually included. The pathological results: F1 to F4 were 8, 13, 14 and 10 respectively, and 11 of the control group were F0.(2) ADC, D^*, and f were lower in the experimental group than in the control group (P<0.01). Spearman analysis showed that ADC, D, f, and D^* were negatively correlated with liver fibrosis levels (P<0.05).(3) The ADC, D, f, D^* values decreased with the progression of hepatic fibrosis, and the ADC of F3 and F4 were significantly different. D^* of F0 and F1, D^* and f of F1 and F2, and D of F3 and F4 were significantly different (P<0.05). The receiver operating characteristic (ROC) curve showed that D^* identified F0 and F1-F4, F0-F1 and F2-F4, and F0-F2 and F3-F4 were better than ADC, D and f. ADC identified F0-F3 and F4 as the most efficient. Conclusions: D^* discriminates F0-F3 with the highest efficiency, and ADC discriminates F0-F3 and F4 with the highest efficacy. D^*, ADC have liver fibrosis staging application value.

关 键 词：肝纤维化 磁共振成像 疾病模型 动物 

分 类 号：R445.2[医药卫生—影像医学与核医学] R575.2[医药卫生—诊断学]