依那西普对糖尿病大鼠视网膜凋亡通路中Fas,TNF-α及caspase-8表达的影响  

Effect of Etanercept on The Expression of Fas, TNF-α, and Caspase-8 in Retinal of Diabetic Rats

作　　者：叶琴[1] 黄焱[2] 朱益华[1] YE Qin;HUANG Yan;ZHU Yihua(Department of Ophthalmology,The First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China;Department of Ophthalmology and Optometry,School of Medical Technology Engineering,Fujian Medical University,Fuzhou 350004,China)

机构地区：[1]福建医科大学附属第一医院眼科,福州350005 [2]福建医科大学医技工程学院视光学系,福州350004

出　　处：《福建医科大学学报》2019年第2期94-97,共4页Journal of Fujian Medical University

基　　金：福建医科大学苗圃重点项目(2015MP004)

摘　　要：目的观察依那西普对糖尿病大鼠视网膜细胞凋亡通路中Fas,TNF-α及caspase-8表达的影响,探讨依那西普对糖尿病视网膜病变的治疗作用。方法SD大鼠随机分为对照组、模型组、治疗组,建立链脲佐菌素(STZ)糖尿病大鼠模型,每周测血糖及体质量。Western-blot法检测大鼠视网膜中Fas,TNF-α及caspase-8的表达,比较各组大鼠视网膜血管伊文思蓝渗漏量。结果治疗组、模型组视网膜的Fas,TNF-α及caspase-8表达均高于对照组(P<0.01),治疗组低于模型组(P<0.01)。治疗组的视网膜伊文思蓝渗漏量低于模型组(P<0.01),治疗组、模型组均高于对照组(P<0.01)。结论依那西普可以有效减少糖尿病大鼠视网膜凋亡通路中Fas,TNF-α及caspase-8的表达,减少糖尿病大鼠视网膜的渗漏量,减轻糖尿病大鼠视网膜病变。Objective To observe the effect of Etanercept on the expression of Fas, TNF-α, and caspase-8 in the retinal apoptotic pathway in diabetic rats, and to explore the therapeutic effect of etanercept on diabetic retinopathy.MethodsSD rats were randomly divided into control group, model group, and treatment group.A streptozotocin (STZ) diabetic rat model was established.The blood glucose and body weight of the model rats were measured every week.Specimens from each group were obtained after 12 weeks of treatment.The expression of Fas, TNF-α, and caspase-8 in rat retina was quantitatively detected by Western-blot, and Evans blue leakage test was used to compare the quantitative determination of retinal vascular leakage in each group of rats.ResultThe expression of Fas, TNF-α, and caspase-8 with Western-blot in the treatment group was lower than that inthe model group ( P <0.01), while in the treatment group and the model group they were higher than that in the control group ( P <0.01 ).The Evans blue leakage test was used to detect the retinal leakage in each group, and it showed the leakage in the treatment group was lower than that in the model group ( P <0.01), while in the treatment group and the model group it were higher than that in the control group ( P <0.01). Conclusion Etanercept can effectively reduce the expression of Fas, TNF-α, and caspase-8 in the retinal apoptotic pathway of diabetic rats, and it can reduce the leakage of retinal cells in diabetic rats, effectively reduce the retinopathy of diabetic rats as well.

关 键 词：糖尿病视网膜病变 抗原 CD95 肿瘤坏死因子α 半胱氨酸天冬氨酸蛋白酶8 

分 类 号：R332[医药卫生—人体生理学] R345.79[医药卫生—基础医学]