培养基配比对‘金踯躅’茎段组培和试管苗生根的影响  

Effects of medium proportion on tissue culture of stem segment and rooting of plantlet of Rhododendron molle ‘Jinzhizhu’

作　　者：田晓玲[1] 刘广超[1] 何娇 路登宇 黄承玲[1] TIAN Xiaoling;LIU Guangchao;HE Jiao;LU Dengyu;HUANG Chengling(The College Humanities & Sciences of Guizhou Minzu University, Guiyang 550025, China;Guizhou Siyuan Agricultural Tourism Comprehensive Development Co., Ltd., Panzhou 553537, China)

机构地区：[1]贵州民族大学人文科技学院,贵州贵阳550025 [2]贵州思源农旅综合开发有限公司,贵州盘州553537

出　　处：《植物资源与环境学报》2019年第2期115-117,共3页Journal of Plant Resources and Environment

基　　金：贵州省科技计划项目(黔科合平台人才[2017]5229)

摘　　要：‘金踯躅’(Rhododendron molle ‘Jinzhizhu’)是园林绿化植物中不可多得的黄色杜鹃花品种,由羊踯躅(Rhododendronmolle G. Don)实生苗开花植株经芽变选育获得,‘金踯躅’的花冠金黄色,与羊踯躅的纯黄色花冠有明显区别。该品种无种子繁殖体,并且扦插繁殖困难。Taking stem segments of one-year-old branch of Rhododendron molle ‘Jinzhizhu’ as explants, and using WPM medium(containing 6.5 g·L^-1 agar, pH 5.6) as basic medium, effects of medium proportion on differentiation of stem segment, proliferation of adventitious bud and rooting of plantlet were studied. The results show that differentiation rate of stem segment is the highest(83.3%) in induction medium(containing 6.5 g·L^-1 agar and 30 g·L^-1 sucrose, pH 5.6) with 1.0 mg·L^-1 2-ip and 0.1 mg·L^-1 NAA, and is significantly higher than that in other media;proliferation rate of adventitious bud is the highest(93.3%) in proliferation medium(containing 6.5 g·L^-1 agar, pH 5.6) with 1.0 mg·L^-1 2-ip, 0.05 mg·L^-1 NAA and 30 g·L^-1 sucrose or 1.5 mg·L^-1 2-ip, 0.10 mg·L^-1 NAA and 20 g·L^-1 sucrose, and proliferation multiple is also bigger. Rooting rate of plantlet is the highest in rooting medium(containing 6.5 g·L^-1 agar and 30 g·L^-1 sucrose, pH 5.6) with 0.50 mg·L^-1 NAA, which reaches 70.0%, but combined addition of NAA and IBA has no evident promotion effect on rooting of R. molle ‘Jinzhizhu’ plantlet.

关 键 词：'金踯躅’ 茎段 组培 激素配比 

分 类 号：S685.21[农业科学—观赏园艺] Q813.12[农业科学—园艺学]