南宁市某医院2014-2017年结核分枝杆菌rpoB、katG和inhA突变监测分析  

Analysis on mutation monitoring of Mycobacterium tuberculosis rpoB,katG and inhA in a hospital in Nanning city from 2014 to 2017

作　　者：韦善求 麻秋英[1] 罗顺达[1] 倪祖彥 文乐敏[2] WEI Shanqiu;MA Qiuying;LUO Shunda;NI Zuyan;WEN Lemin(Clinical Laboratory the Fourth People's Hospital of NanningCity,Nanning,Guangxi 530023,China;Medical Department,the Fourth People's Hospital of NanningCity,Nanning,Guangxi 530023,China)

机构地区：[1]广西壮族自治区南宁市第四人民医院检验科,530023 [2]广西壮族自治区南宁市第四人民医院医务部,530023

出　　处：《重庆医学》2019年第10期1709-1712,共4页Chongqing medicine

基　　金：广西壮族自治区南宁市科学研究与技术开发计划项目(20143146)

摘　　要：目的了解某院结核分枝杆菌rpoB、katG和inhA突变特征及耐药性。方法采集经聚合酶链反应(PCR)鉴定为结核分枝杆菌的标本2 624份,采用线性探针(LipA)法检测rpoB、katG和inhA突变。结果 2 072份结果有效,rpoB、katG及inhA突变率依次是14.77%、12.11%及2.80%;相关利福平、异烟肼耐药率及耐多药率为14.77%、14.62%、9.60%。异烟肼耐药株中katG 315位突变占82.84%,rpoB突变株中突变频率居前的位点是531(63.40%)、H526Y或H526D(11.11%)、其他H526(8.50%),突变类型中"突变型探针出现并对应野生型探针缺失"占81.37%。结论该院结核分枝杆菌rpoB、katG和inhA突变特征与国内文献报道相近,相关利福平、异烟肼耐药率及耐多药率高于广西平均水平。Objective To understand the mutation characteristics of Mycobacterium tuberculosis rpoB,katG and inhA genes and drug resistance in our hospital.Methods A total of 2 624 samples identified by PCR as Mycobacterium tuberculosis were collected.The mutation in rpoB,katG,and inhA genes were determined by Liner probe Assay(LipA).Results The results of 2 072 cases were valid.The mutation rate of rpoB,katG,and inhA was 14.77%,12.11%,and 2.80%,respectively.The drug resistance rate of rifampicin,isoniazid and multi-drug resistance rate was 14.77%,14.62%,and 9.60%,respectively.Among the isoniazid-resistant mutants,the katG 315 mutation accounted for 82.84%.And among the rpoB mutants the top three mutation sites were 531(63.40%)、H526 Yor H526 D(11.11%),and other H526(8.50%)location.In the mutation type," mutant probes appearing and corresponding wild type missing" accounted for 81.37%.Conclusion The mutation characteristics of rpoB,katG,and inhA genes in Mycobacterium tuberculosis identified in this hospital are similar to those reported in the domestic literature.The related drug resistance rates of rifampicin,isoniazid and multi-drug are higher than the average rate in Guangxi region.

关 键 词：广泛耐药结核 结核分枝杆菌 rpoB/katG/inhA 耐药 线性探针技术 

分 类 号：R378.91[医药卫生—病原生物学]