壮医药线点灸对糖尿病性胃轻瘫大鼠脑肠肽Ghrelin、CCK的影响  

Effects of medicated thread moxibustion of Zhuang medicine on brain-gut peptide Ghrelin and CCK in diabetic gastroparesis rats

作　　者：张勇 李蓓蕾[1] 梁修朗 唐莫玲 黄梓健 范郁山[2] ZHANG Yong;LI Beilei;LIANG Xiulang;TANG Moling;HUANG Zijian;FAN Yushan(Graduate School,Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China;College of Acupuncture and Massage,Guangxi University of ChineseMedicine,Nanning,Guangxi 530001,China)

机构地区：[1]广西中医药大学研究生院,南宁530001 [2]广西中医药大学针灸推拿学院,南宁530001

出　　处：《重庆医学》2019年第9期1457-1461,共5页Chongqing medicine

基　　金：中国中医科学院自主选题项目(ZZKF06015);2017级研究生创新计划资助项目(YJSZ201709;YJSZ201707)

摘　　要：目的观察壮医药线点灸对糖尿病性胃轻瘫(DGP)大鼠脑肠肽胆囊收缩素(CCK)、促生长素(Ghrelin)水平的影响。方法将80只SD大鼠分为空白组(生理盐水灌胃)、模型组(生理盐水灌胃)、壮医药线点灸组(壮医药线点灸中脘、内关、足三里、脾俞、胃俞穴)和甲氧氯普胺组(甲氧氯普胺药液灌胃),每组20只。除空白组外,其余3组大鼠均采用链脲佐菌素(STZ)配合高糖高脂饲料诱导造模。各组大鼠分别于相应措施干预15d后,检测血糖、胃排空率、小肠推进率,ELISA法检测血清Ghrelin水平及胃窦CCK水平,Western blot法检测胃窦Ghrelin的蛋白表达。结果与空白组比较,模型组大鼠胃肠排空情况、Ghrelin血清水平及胃窦表达水平明显降低(P<0.05),而血糖、胃窦CCK水平升高(P<0.05);与模型组比较,壮医药线点灸组胃肠排空情况和血清及胃窦Ghrelin表达水平均明显升高(P<0.05),而血糖、胃窦CCK水平均降低(P<0.05);与甲氧氯普胺组比较,壮医药线点灸组胃肠排空情况相似,余各项指标均优于甲氧氯普胺组(P<0.05)。结论壮医药线点灸能促进胃肠排空从而改善DGP症状,其作用机制可能与其上调血清Ghrelin水平和下调胃窦CCK水平,并促进Ghrelin在胃窦部蛋白表达相关。Objective To observe the effects of medicated thread moxibustion of Zhuang medicine(MTMZ)on the levels of the brain-gut peptides Ghrelin and cholecystokinin(CCK)in rats with diabetic gastroparesis(DGP).Methods 80 SD rats were randomly divided into 4 groups:the normal group(intragastric gavage with saline),the DGP model group(intragastric gavage with saline),the MTMZ group [MTMZ applied at Zhongwan(CV 12),Neiguan(PC 6),Zusanli(ST 36),Pishu(BL 20)and Weishu(BL 21)points],the metoclopramide group(gavage with metoclopramide),with 20 cases in each group.The DGP model was established by intraperitoneal injection of Streptozotocin(STZ)combining with a high-glucose/high-fat diet for 8 weeks.After 15 days,blood glucose,gastric evacuation rate and small intestinal propulsive rate were measured.Ghrelin in serum and CCK in gastric antrum were determined by ELISA.The expression of Ghrelin in gastric antrum was detected by Western Blot.Results Compared with the normal group,gastric evacuation rate,Ghrelin expression decreased significantly in the DGP model group,whereas blood glucose and CCK level significantly increased.Meanwhile,compared with the DGP model group,gastric evacuationrate,Ghrelin expression increased and blood glucose,CCK decreased significantlyin in the MTMZ group(P<0.01).The MTMZ group was similar to the metoclopramide group in gastric evacuationrate,while the other indexes were better.Conclusion MTMZ can promote gastro-intestinal emptying,and relieve DGP symptoms.Its underlying mechanism may be through raising serum Ghrelin and lowering gastric antrum CCK,promoting the expressions of Ghrelin related proteins in gastric antrum.

关 键 词：糖尿病性胃轻瘫 壮医药线点灸 脑肠肽 促生长素 胆囊收缩素 大鼠 Sprague-Dawley 

分 类 号：R29[医药卫生—民族医学]