HPLC法测定芩杏清热止咳颗粒中8种成分  

Determination of eight constituents in Qinxing Qingre Zhike Granules by HPLC

作　　者：王子凌云 梁琨[1] 李国文 WANG Zi-ling-yun;LIANG Kun;LI Guo-wen(1.Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属上海市中西医结合医院,上海200082

出　　处：《中成药》2019年第5期995-999,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法测定芩杏清热止咳颗粒(黄芩、栀子、桑叶等)中8种成分的含有量。方法黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的分析采用Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相0.2%磷酸-甲醇,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长274 nm。栀子苷、甘草苷、异甘草苷、甘草酸的分析采用Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相0.05%磷酸-乙腈,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长237、360 nm。结果 8种成分在各自范围内线性关系良好(r>0.999 5),平均加样回收率97.35%～98.73%,RSD 0.61%～1.9%。结论该方法准确可靠,重复性好,可用于芩杏清热止咳颗粒的质量控制。AIM To establish an HPLC method for the content determination of eight constituents in Qinxing Qingre Zhike Granules(Scutellariae Radix,Gardeniae Fructus,Mori Folium,etc.).METHODS The analyses of baicalin,wogonoside,baicalein and wogonin were performed on a 25℃thermostatic Diamonsil Plus C18column(4.6 mm×250 mm,5μm),with the mobile phase comprising of 0.2%phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 274 nm.The analyses of geniposide,liquritin,isoliquiritin and glycyrrhizic acid were performed on a 25℃thermostatic Diamonsil Plus C18column(4.6 mm×250 mm,5μm),with the mobile phase comprising of 0.05%phosphoric acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,360 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 5),whose average recoveries were 97.35%-98.73%with the RSDs of 0.61%-1.9%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Qinxing Qingre Zhike Granules.

关 键 词：芩杏清热止咳颗粒 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]