胡桃醌通过线粒体途径抑制宫颈癌Hela细胞生长的研究  

Inhibition effect of Juglone on cervical cancer Hela cell growth through mitochondrial pathway

作　　者：夏荣钧[1] 邵雅楠 张锦辉[1] 刘新[1] 姜瑜[1] 王研[1] 于生金[1] 林黎娟[1] XIA Rong-jun;SHAO Ya-nan;ZHANGJin-hui;LIU Xin;JIANG Yu;WANG Yan;YU Sheng-jin;LIN Li-juan(School of Medicine, Eastern Liaoning University, Dandong 118003,China)

机构地区：[1]辽东学院医学院,辽宁丹东118003

出　　处：《辽东学院学报（自然科学版）》2019年第2期97-101,共5页Journal of Eastern Liaoning University:Natural Science Edition

基　　金：2016辽东学院科研基金社发攻关项目(2016YY035);2017辽东学院科研基金青年项目(2017QN043);2017中央财政专项平台建设项目;2018中央财政专项团队建设项目;2018辽宁省科技厅指导项目(20180550356)

摘　　要：胡桃醌的化学成分为5-羟基-1,4-萘二酮,是从核桃楸中分离出的重要化合物,对多种恶性肿瘤的生长具有抑制活性。研究中,子宫颈癌Hela细胞接受不同剂量的胡桃醌处理,利用MTT实验检测胡桃醌对Hela细胞的增殖抑制作用、流式细胞术检测胡桃醌对Hela细胞周期和凋亡的影响以及Western blot检测CytC、Bcl-2、Bax和caspases蛋白水平的表达。结果表明,胡桃醌可以呈剂量依赖性的方式显著抑制Hela细胞的活性。此外,膜联蛋白Annexin V-FITC在50μM和100μM浓度的早期凋亡率分别为16. 79%和30. 03%,而对照组为10. 73%。不同剂量胡桃醌处理Hela细胞后,Cytc水平和Bax/Bcl-2比值较对照组明显增高,同时caspase-3、-9的活性也明显变化。结果表明,胡桃醌可能通过线粒体途径诱导细胞凋亡有效抑制Hela细胞的生长。The chemical component of juglone is 5-hydroxy-1,4- naphthalenedione, which is major chemical constituent of Juglans mandshurica Maxim and confirmed to possesses anti-tumor activity on the growth of a variety of malignant tumors.In this study,cervical cancer Hela were cultured and treated with different dosages of juglone.MTT assay was performed to examine the proliferation inhibition of juglone on Hela cells.Flow cytometry was used to study juglone on the cell cycle and apoptosis of juglone-treated Hela cells. The protein expression levels of CytC, Bcl-2, Bax and caspases were measured by Western blot.The results showed that juglone significantly inhibited Hela cells survival in a dose-dependent manner.Moreover, the percentages of early apoptosis of Annexin V-FITC were16.79% and30.03% in 50 μM and100 μM groups, compared with 10.73% in the control group, respectively.After cells were treated at different doses, juglone increased Cytc level and Bax/Bcl-2 ratio significantly compared with those of the control group.These events paralleled with activation of caspase-3and caspase-9.The results suggest that juglone may be effective for the treatment of Hela cells and probably induce apoptosis through mitochondrial pathway.

关 键 词：胡桃醌 HELA细胞 凋亡 线粒体途径 

分 类 号：R932[医药卫生—生药学] R737[医药卫生—药学]