补益肾气法干预不同周龄自发性高血压大鼠大动脉管壁结构的组织形态学研究  

Study on Effect of Tonifying Kidney-Qi Methodon Arteriosclerosis in Difference Aging SHR

作　　者：于杰[1] 张磊[2] 赵书颖 YU Jie;ZHANG Lei;ZHAO Shuying(Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;The Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东中医药大学附属医院,山东济南250014

出　　处：《中华中医药学刊》2019年第5期1208-1211,I0029-I0031,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：山东省自然科学基金项目(ZR2015PH051);中西医结合心血管泰山学者工程专项项目

摘　　要：目的:通过观察不同周龄自发性高血压大鼠(SHR)经补益肾气法干预前后大动脉的组织形态学变化,评价补益肾气法对高血压病大动脉管壁结构的保护作用。方法:标准尾部测压法测量尾动脉收缩压;苏木素-伊红(HE)染色法对比观察动脉管壁形态结构变化;透射电镜法观察各大动脉的胶原排列情况。结果:(1)血压结果:模型对照组SHR大鼠随着周龄的增加,收缩压不断上升,差异非常明显。补肾中药组SHR和氨氯地平组SHR大鼠随着周龄的增加,收缩压不断下降,差异明显。正常对照组WKY大鼠随着周龄的增加,收缩压变化较小,差异不明显。(2)苏木素-伊红(HE)染色结果:正常组大鼠胸主动脉、颈动脉管壁的形态结构均大致正常;模型对照组SHR胸主动脉、颈动脉出现不同程度的血管重构情况,血管损伤程度随周龄增加呈逐渐加重的趋势;补肾中药组SHR与氨氯地平组SHR胸主动脉、颈动脉也存在血管重构表现,血管损伤情况也存在随增龄逐渐加重现象,但是与同龄模型对照组SHR相比血管损伤情况明显减轻;同龄补肾中药组SHR与氨氯地平组SHR比较,血管损伤的差异不明显。(3)透射电镜结果:正常组的主动脉及颈动脉的血管壁细胞结构完整、形态正常;补肾中药组、氨氯地平组和模型对照组的主动脉、颈动脉可见不同程度的血管壁细胞结构损伤。补肾中药组和氨氯地平组SHR胸主动脉、颈动脉血管损伤情况存在随增龄逐渐加重现象,但是与同龄模型对照组相比血管损伤情况明显减轻;同龄补肾中药组与氨氯地平组SHR比较,血管损伤的差异不明显。结论:随着年龄增加,SHR大鼠动脉组织形态变化明显,动脉硬化呈逐渐加重趋势,通过补益肾气药物干预,SHR动脉硬化程度可见明显减轻,补益肾气法可以在降压的同时保护大动脉管壁,减轻动脉组织形态学改变的程度,有效延缓动脉硬化的进程。Objective:To observe the changes of arterial tissue morphology in difference aging SHR and explore the effect of tonifying kideny-Qi method on atherosclerosis in hypertensive patients.Methods:Standard tail pressure measurement was used to measure the systolic blood pressure.The morphological structure of arterial wall was observed by HE staining method.The collagen arrangement of the aorta was observed by TEM method.Result:(1)In the model control group,the systolic blood pressure of SHR increased with the week aging,which was statistically significant(P<0.01) compared with the elderly rats in the previous week. After treatment,the systolic blood pressure of SHR in the Chinese medicine group was significantly lowered with statistical difference(P<0.05).(2)The systolic blood pressure of amlodipine group was decreased significantly after 12 weeks treatmentand the difference was statistically significant(P<0.05).The morphological structure of the thoracic aorta and carotid artery wall in the normal group were normal.SHR thoracic aorta and the carotid artery of the model control group appearred different degrees of vascular remodeling, aortic intima hyperplasia or falling off, irregularmembrane elastic fibers or rupture, smooth muscle cells disordered arrangement and different degrees of hyperplasia. The vascular damage degree increased with weeks and showed a trend of gradual increase. Chinese medicine group and amlodipine group’s thoracic aorta and the carotid artery had vascular remodeling. The vascular injury situation was also gradually increased along with aging. But compared with the peer model control group,the blood vessel damage situation was significantly reduced. Compared with the amlodipine group, the differences in vascular injury were not obvious.The wall cells of the aorta and carotid artery in the normal group were intact and normal. Traditional Chinese medicine group, amlodipine group and model control group’s aorta and carotid artery wall were thickening and the endothelial cells had different d

关 键 词：补益肾气法 高血压病 动脉硬化 HE染色 透射电镜法 SHR大鼠 

分 类 号：R285.5[医药卫生—中药学]