猪流行性腹泻病毒SHpd/2012株Nsp8基因分析及真核表达  

Gene analysis and eukaryotic expression of Nsp8 in strain SHpd/2012 of porcine epidemic diarrhea virus

作　　者：丁思嘉[1] 罗依然[1] 周书亭 马世文 蒲良 杨亮 刘源平 蒋升瑶 叶力波利.达吾力 杨志彪[1] DING Si-jia;LUO Yi-ran;ZHOU Shu-ting;MA Shi-wen;PU Liang;YANG Liang;LIU Yuan-ping;JIANG Sheng-yao;YELIBOLI Dawuli;YANG Zhi-biao(Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海200240

出　　处：《上海交通大学学报（农业科学版）》2019年第2期64-69,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家重点研发计划(2016YFD0500100);国家自然科学基金(31472211)

摘　　要：应用生物信息工具和分子克隆技术对PEDV SHpd/2012株Nsp8基因编码的蛋白进行了结构和功能分析以及体外表达。结果显示,NSP8蛋白是一个含有194个氨基酸的亲水性蛋白,不含信号肽和跨膜区。SHpd/2012毒株高度保守的NSP8氨基酸序列与CV777、AJ1102等毒株同源性高。NSP8蛋白也是一个单体蛋白,共有5个优势抗原表位。此外,本研究成功地在293T细胞内表达了NSP8蛋白,为进一步研究猪流行性腹泻病毒NSP8蛋白的功能提供了理论基础。Bioinformatics tools were used to study the structure and function of NSP8 protein in strain SHpd/2012 of porcine epidemic diarrhea virus(PEDV).The results show that NSP8 is a hydrophilic protein,which contains 194 amino acids,without signal peptide and transmembrane region.Its conserved amino acid sequence is highly homologous to strains CV777 and AJ1102.Furthermore,the results also show that NSP8 is a monomeric protein with five dominant epitopes and can be successfully expressed in 293T cells,which provides a theoretical basis for further study of the function of PEDV NSP8.

关 键 词：猪流行性腹泻病毒 Nsp8基因 真核表达 

分 类 号：S858.28[农业科学—临床兽医学]