低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响  

Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells

作　　者：张金友[1] 黄鹤[2] 李伟[1] 韩欢胜[1] 甘文平[1] 宋伟红[1] 卫喜明[1] ZHANG Jinyou;HUANG He;LI Wei;HAN Huansheng;GAN Wenping;SONG Weihong;WEI Ximing(Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Land Reclamation Science,Harbin 150038,China;Heilongjiang Provincial Center for Disease Control and Prevention,Harbin 150030,China)

机构地区：[1]黑龙江省农垦科学院畜牧兽医研究所,哈尔滨150038 [2]黑龙江省疾病预防控制中心,哈尔滨150030

出　　处：《中国畜牧兽医》2019年第5期1525-1531,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家奶牛产业技术体系哈尔滨农垦综合试验站(CARS-36);规模化奶牛场均衡增产关键技术集成创新与示范(HNK135-04-01)

摘　　要：本研究旨在探讨低葡萄糖水平对奶牛乳腺上皮细胞中肿瘤坏死因子-α(TNF-α)、溶菌酶(LYZ)、诱导型一氧化氮合酶(iNOS)、白细胞介素-6 (IL-6)、乳铁蛋白(LF)和白细胞介素-8(CXCL8)等促炎症因子mRNA表达的影响。采用酶消化法将奶牛乳腺上皮细胞分离纯化后,分别在含有0.25、1.00和4.50 mg/mL葡萄糖的完全培养液中培养24 h,采用实时荧光定量PCR技术检测细胞中相关基因的表达。结果表明,上述基因的mRNA相对表达量在各葡萄糖浓度之间没有明显差异(P>0.05)。其中TNF-α和LYZ在各浓度葡萄糖中的表达量均非常少,iNOS的表达量居中,而IL-6、LF和CXCL8在各浓度组中的表达量相对较高。试验结果提示,葡萄糖浓度的减少并没有直接影响乳腺上皮细胞中TNF-α、LYZ、iNOS、IL-6、LF和CXCL8 mRNA的基础性表达;乳腺上皮细胞在未受到感染的情况下,能够表达一定数量的iNOS、IL-6、LF和CXCL8,这些因子可以作为先天性免疫防御的储备,在受到感染的情况下能迅速应对病菌的入侵。The aim of this study was to investigate the effects of low level glucose on mRNA expression of tumor necrosis factor-α(TNF-α),lysozyme (LYZ),induced nitric oxide synthase (iNOS),interleukin-6 (IL-6),lactoferrin (LF) and interleukin-8 (CXCL8) in bovine mammary epithelial cells.After enzymatic digestion,the bovine mammary epithelial cells were isolated and purified,and then cultured in complete medium containing 0.25,1.00 and 4.50 mg/mL glucose respectively.The expressions of related genes in cells were detected by Real-time fluorescence quantitative PCR.The results showed that there were no significant differences in the relative mRNA expression of the above genes among three glucose groups ( P >0.05).The expression levels of TNF-α and LYZ in each concentration group were very low.The expression levels of iNOS were in the middle.However,the expression of IL-6,LF and CXCL8 in different concentration groups was relatively high.It suggested that the decrease of glucose concentration did not directly affect the basic expressions of TNF-α,LYZ,iNOS,IL-6,LF and CXCL8 mRNA in mammary epithelial cells.While without infection,mammary epithelial cells could express a certain amount of iNOS,IL-6,LF and CXCL8,which could be used as a reserve for innate immune defense in order to quickly respond to the invasion of pathogens.

关 键 词：奶牛 乳腺上皮细胞 葡萄糖 炎症 

分 类 号：S823[农业科学—畜牧学]