健康妊娠与子痫前期患者循环微粒体携带蛋白差异分析  

Circulating microsome proteins in the pathogenesis of preeclampsia

作　　者：张云聪 杨静[2] 杨硕[3] 崔婵娟 赵诚[2] 郭晗 赵扬玉[2] 张捷[3] 魏瑗[2] 乔蕊[3] Zhang Yuncong;Yang Jing;Yang Shuo;Cui Chanjuan;Zhao Cheng;Guo Han;Zhao Yangyu;Zhang Jie;Wei Yuan;Qiao Rui(Department of Clinical Laboratory, Peking University International Hospital, Beijing 102206, China;Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China;Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China;Department of Clinical Laboratory, Cancer Hospital Chinese Academy of Medical Science, Beijing 100021, China)

机构地区：[1]北京大学国际医院检验科,北京102206 [2]北京大学第三医院妇产科,北京100191 [3]北京大学第三医院检验科,北京100191 [4]国家癌症中心/中国医学科学院北京协和医学院肿瘤医院检验科,北京100021

出　　处：《中华检验医学杂志》2019年第4期255-261,共7页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金(81601824);北京大学第三医院院临床重点项目(BYSY2017008)。

摘　　要：目的研究微粒水平及其蛋白质在子痫前期时的变化,初步探索微粒在子痫前期发生和进展中的作用机制。方法2016年12月至2018年6月,在北京大学第三医院连续入选98例子痫前期患者、54名健康妊娠女性和51名健康育龄女性,应用流式细胞术检测其MPs水平,蛋白质谱检测微粒所携带蛋白质表达情况。结果健康妊娠组总MPs水平较健康育龄女性组明显升高[159.87(113.25,218.18)/μl和94.10(53.35,140.23)/μl,P=0.004],但与子痫前期组比无明显差别(P>0.05)。提取各组微粒进行蛋白质谱分析,健康妊娠女性与健康育龄女性之间有30种差异蛋白,与凝血级联反应、补体和其他免疫反应、血管生成等生物学过程联系密切;子痫前期患者与健康妊娠女性之间有14种差异蛋白,与凝血级联反应、补体和炎症反应以及血管生成等生物学过程联系密切。结论循环微粒数量变化可以反映子痫前期的高凝状态。循环微粒可能通过所携蛋白质经多种途径参与子痫前期发病机制,对子痫前期干预研究具有重要潜在价值。Objective In this study, we aimed to detect the level of total circulating microparticles (MPs) in pregnant women with preeclampsia (PE) and analyze the proteome of MPs to explore their roles in the pathogenesis and progression of PE. Methods 98 pregnant women with PE, 54 healthy pregnant women, and 51 healthy non-pregnant women were enrolled from December 2016 to June 2018, whose MP levels were detected by flow cytometry and compared. Proteins extracted from the MPs were analyzed by high performance liquid chromatography mass spectrometry. Results The total MP level of the healthy pregnant group was significantly higher than thatof the non-pregnant group [159.87 (113.25, 218.18)/μl vs 94.10 (53.35, 140.23)/μl, P=0.004], but was not significantly different from that of the PE group. By proteomic profiling, 30 differential proteins were obtained between healthy pregnant women and healthy non-pregnant women, which were closely related to biological processes such as complements, coagulation cascades, angiogenesis and so on;14 differential proteins were found between PE patients and healthy pregnant women, which were closely related to biological processes such as coagulation cascades, complements and inflammatory reactions, angiogenesis and so forth. Conclusions The level of circulating MPs may reflect the hypercoagulability of preeclampsia. In addition, circulating MPs may be involved in the pathogenesis of PE through various pathways by carrying different proteins, which indicates their potential value in the intervention of PE.

关 键 词：先兆子痫 细胞源性微粒 血栓形成倾向 蛋白质组学 

分 类 号：R446.1[医药卫生—诊断学] R714.244[医药卫生—临床医学]