温度和pH对棕黑锦蛇消化酶活性的影响  

Effects of Temperature and pH on Digestive Enzyme Activities in Elaphe schrenckii

作　　者：金建丽[1] 刘思宇[1,2] 刘艳爽 曹明星 JIN Jianli;LIU Siyu;LIU Yanshuang;CAO Mingxing(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011;Fujin Fourth Middle School,Fujin,Heilongjiang 156100,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]富锦市第四中学,黑龙江富锦156100

出　　处：《贵州农业科学》2019年第5期80-85,共6页Guizhou Agricultural Sciences

基　　金：牡丹江师范学院研究生科技创新项目(kjcx2017-06mdjnu);(kjcx2017-24mdjnu)

摘　　要：为对棕黑锦蛇的人工养殖及深入研究提供参考,采用酶学分析方法研究pH(5.0、5.4、5.8、 6.2 、6.6、7.0、7.4、7.8、8.2、8.6、9.0、9.4、9.8、10.2、10.6)和温度(23℃、26℃、29℃、32℃、35℃、38℃、41℃、44℃、47℃、50℃、53℃、56℃)对棕黑锦蛇消化酶活性的影响。结果表明:棕黑锦蛇食道、胃、小肠、大肠、肝脏、胰脏中淀粉酶活力的最适pH分别是6.6、6.2、6.6、6.6、7.0和5.8,蛋白酶活力的最适pH分别是9.8、6.6、9.0、8.6、5.8和10.2,脂肪酶活力的最适pH分别是6.2、5.4、5.8、6.6、7.4和5.8。棕黑锦蛇食道、胃、小肠、大肠、肝脏、胰脏中淀粉酶活力的最适温度分别是32℃、35℃、35℃、35℃、38℃和29℃;蛋白酶活力的最适温度分别是38℃、41℃、38℃、44℃、47℃和53℃;脂肪酶活力的最适温度分别是38℃、47℃、38℃、35℃、41℃和44℃。在最适pH和温度条件下,棕黑锦蛇消化系统6个组织中淀粉酶活力胰脏>食道>肝脏>胃>小肠>大肠,且差异显著( P<0.05);蛋白酶活力小肠>肝脏>胰脏>食道>胃>大肠,且差异显著(P<0.05);脂肪酶活力胰脏>肝脏>小肠>胃>食道>大肠,且差异显著(P <0.05)。In order to provide references for the artificial breeding and in-depth study of Elaphe schrenckii , enzymatic analysis method was employed to explore the effects of pH(5.0, 5.4, 5.8, 6.2, 6.6 , 7.0, 7.4, 7.8, 8.2, 8.6, 9.0, 9.4, 9.8, 10.2, 10.6) and temperature (23℃, 26℃, 29℃, 32℃, 35℃, 38℃, 41℃, 44℃, 47℃, 50℃, 53℃, 56℃) on digestive enzyme activities of E. schrenckii . Results: The optimum pH values for amylase activity in the esophagus, stomach, small intestine, large intestine, liver and pancreas of E. schrenckii were 6.6, 6.2, 6.6, 6.6, 7.0 and 5.8, respectively, for protease activity they were 9.8. 6.6, 9.0, 8.6, 5.8, and 10.2 respectively;and for lipase activity they were 6.2, 5.4, 5.8 , 6.6, 7.4 and 5.8, respectively. The optimal temperature for amylase activity in esophagus, stomach, small intestine, large intestine, liver and pancreas of E. schrenckii were respectively 32℃, 35℃, 35℃, 35℃, 38℃ and 29℃, for protease activity they were 38℃, 41℃, 38℃, 44℃, 47℃ and 53℃, respectively, and for lipase activity they were 38℃, 47℃, 38℃, 35℃, 41℃ and 44℃, respectively. Under optimal pH and temperature conditions, the amylase activity in the six tissues of E. schrenckii digestive system was pancreas>esophagus>liver>stomach>small intestine>large intestine, and the difference was significant ( P <0.05);Protease activity followed the sequence of small intestine>liver > pancreas > esophagus > stomach > large intestine, and the difference was also significant ( P <0.05);Lipase activity was of the sequence of pancreas > liver > small intestine > stomach > esophagus > large intestine, and the difference was significant ( P <0.05) too.

关 键 词：棕黑锦蛇 酶学分析 最适温度 最适PH 消化酶 酶活力 

分 类 号：S864[农业科学—野生动物驯养]