HPLC同时测定五味子中7个木脂素类化合物的含量~△  

HPLC Simultaneous Determination of 7 Major Dibenzocyclooctadiene-type Lignans in fruits of Schisandrae chinensis

作　　者：王书云 梁宏刚[2,3] 黄健 王金辉[2,3] 杨宝峰[3] WANG Shu-yun;LIANG Hong-gang;HUANG Jian;WANG Jin-hui;YANG Bao-feng(School of Pharmacy,Henan University,Kaifeng 475004,China;Department of Medicinal Chemistry and Natural Medicine Chemistry (State-Province Key Laboratories ofBiomedicine-Pharmaceutics of China,Harbin Medical University,Harbin 150081,China;School of Traditional Chinese Materia Medica,Shenyang PharmaceuticalUniversity,Shenyang 110016,China)

机构地区：[1]河南大学药学院,河南开封475004 [2]哈尔滨医科大学药化教研室/省部共建生物医药工程重点实验室,黑龙江哈尔滨150081 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国现代中药》2019年第4期473-477,共5页Modern Chinese Medicine

基　　金：国家重大新药创制(2018ZX09305005);护肝片标准化建设(ZYBZH-C-HLJ-15);博士科研启动基金(CJ3050A24051)

摘　　要：目的:建立同时测定五味子中五味子醇甲、戈米辛D、五味子醇乙、当归酰戈米辛H、五味子甲素、戈米辛N、五味子乙素含量的HPLC方法。方法:采用SunFire^(TM) C_(18)色谱柱(150 mm×4.6 mm,5μm),乙腈-水梯度洗脱,流速:1 mL·min^(-1),检测波长254 nm,柱温:30℃。结果:7个化合物在各自范围内均呈良好的线性关系,平均加样回收率为98.23%～100.51%,RSD为0.68%～1.80%。发现不同产地五味子中化合物的含量差别不大,7个木脂素的总质量分数最高为16.27 mg·g^(-1),最低为9.52 mg·g^(-1)。结论:本实验建立的HPLC含量测定方法准确、可靠、重复性好,可用于五味子的质量控制。Objective: To establish a method for the simultaneous determination of 7 dibenzocyclooctadiene-type lignans in Fructus Schisandrae Chinensis. Methods: The analysis was performed on a SunFire TM C 18 column ( 150 mm×4.6 mm,5 μm )at 30°C,with the mobile phase acetonitrile-water system in a gradient elution manner at a flow rate of 1.0 ml/min,and the detection wavelength was set at 254 nm. Results: The linear range of the seven dibenzocyclooctadiene-type lignans were good in their own ranges,and their average recovery were between 98.23% and 100.51% with the RSDs of 0.68%-1.80%.There was no significant difference of the contents of seven compounds in Fructus Schisandrae Chinensis from different batches.The total content of seven investigated lignans was among 9.52-16.27 mg · g ^-1 . Conclusions: The method is accurate,convenient and reproducible for the determination of 7 dibenzocyclooctadiene-type lignans in Fructus Schisandrae Chinensis.

关 键 词：五味子 联苯环辛烯型木脂素类化合物 高效液相色谱法 含量测定 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]