大卫颗粒的质量标准提高  

Improvement of Quality Standard of Dawei Granules

作　　者：李晓[1,2] 王学方 张丽先[1,2] 李飞飞 宁二娟[1,2] 魏悦 LI Xiao;WANG Xue-fang;ZHANG Li-xian;LI Fei-fei;NING Er-juan;WEI Yue(Biotechnology Developing Center of Henan Academy of Sciences,Zhengzhou450002,China;Henan Plant Natural Products Development Engineering Technology Center,Zhengzhou 450002,China)

机构地区：[1]河南省生物技术开发中心,河南郑州450002 [2]河南省植物天然产物开发工程技术研究中心,河南郑州450002

出　　处：《中国现代中药》2019年第4期517-522,共6页Modern Chinese Medicine

基　　金：河南省科技攻关项目(152102110115);河南省科学院科研开发项目(18KF13003)

摘　　要：目的:提高大卫颗粒的质量标准。方法:对制剂中连翘、金银花、柴胡及甘草进行薄层色谱(TCL)鉴别;采用高效液相色谱法(HPLC)同时测定制剂中绿原酸、连翘酯苷A、黄芩苷的含量。结果:连翘、金银花、柴胡及甘草的TLC图斑点清晰,分离度好,阴性对照无干扰。绿原酸、连翘酯苷A、黄芩苷检测质量浓度线性范围分别为5.228～52.28(r=0.999 9)、5.92～59.20(r=0.999 8)、6.816～68.16μg·mL^(-1)(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;平均加样回收率分别为101.84%(RSD=0.44%,n=6)、99.07%(RSD=0.79%,n=6)、101.30%(RSD=0.62%,n=6)。结论:该研究所建标准定性定量方法简便、专属性强、结果准确可靠,可用于大卫颗粒的质量控制。Objective: To improve the quality standard of Dawei granules. Methods: TLC was applied for qualitative identification of Forsythia suspensa , Lonicerae japonicae , Bupleurum chinense , Glycyrrhiza uralensis .The contents of baicalin,chlorogenic acidand and forsythoside A were determined by HPLC. Results: TLC spots of F.suspensa , L.japonicae , B.chinense and G.uralensis were clear and well-separated without negative interference.The linear ranges of chlorogenic acidand,forsythoside A and baicalin were 5.228- 52.28 μg · mL ^-1 ( r= 0.999 9 ),5.92-59.20 μg · mL^-1 ( r= 0.999 8 ) and 6.816-68.16 μg · mL^-1 ( r= 0.999 9 ).RSDs of precision,stability and reproducibility tests were all lower than 1.0 %.The average sample adding recovery rates of chlorogenic acidand,forsythoside A and baicalin were 101.84 %(RSD=0.44%, n = 6 ),99.07 %(RSD=0.79%, n =6 )and 101.30 %(RSD=0.62%, n =6 ),respectively. Conclusions: This method is accurate, sensitive,simple and specific in good repeatability,which is suitable for quality control of Dawei granules.

关 键 词：大卫颗粒 薄层色谱鉴别 含量测定 质量控制 

分 类 号：R286[医药卫生—中药学] R944.26[医药卫生—中医学]