牛粪好氧堆肥中真菌群落组成的动态特征  

Dynamic characteristics of fungal community composition in aerobic cow manure compost

作　　者：许修宏[1] 门梦琪 孟庆欣[1] 许本姝 盛思远 武晓桐 姜欣 韩悦 朱海峰 邓利廷 XU Xiuhong;MEN Mengqi;MENG Qingxin;XU Benshu;SHENG Siyuan;WU Xiaotong;JIANG Xin;HAN Yue;ZHU Haifeng;DENG Liting(School of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2019年第4期45-53,共9页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(31672469)

摘　　要：采用高通量测序技术,探究牛粪堆肥过程中真菌群落时空动态变化、营养型及理化参数对真菌群落结构组成影响。结果表明,牛粪堆肥中未分类子囊菌门(Ascomycota)在升温期相对丰度最高,鬼伞属(Coprinus)和Guehomyces为高温期优势菌群,未分类子囊菌门为降温期优势菌属,未分类散囊菌目(Eurotiales)和Zopfiella为腐熟期优势菌群。在空间层次上,Guehomyces和未分类散囊菌目为上层和中层优势菌群,鬼伞属(Coprinus)和Zopfiella为下层优势菌群。在牛粪堆肥过程中腐生真菌相对丰度逐渐升高,堆肥下层相对丰度高于上、下两层。冗余分析(RDA)表明,含水率和温度对真菌群落结构变化影响显著(P<0.05)。牛粪堆肥过程中真菌群落结构组成在不同时期和空间上均存在差异,堆肥内真菌群落多数为腐生营养型,真菌群落结构变化与理化因子相关。High-throughput sequencing was used to study the temporal and spatial dynamics of fungal community and its guilds during composting of cow manure,and the effects of physical and chemical parameters on the structure of fungal community.The results showed that the relative abundance of unclassified Ascomycota was the highest during heating stages,Coprinus and Guehomyces were the dominant fungi in high temperature stages,unclassified Ascomycota was the dominant fungi in cooling stages,unclassified Eurotiales and Zopfiella were the dominant fungi in maturity stages.At the spatial level,Guehomyces and unclassified Eurotiales were dominant fungi in the top and middle piles,while Coprinus and Zopfiella were dominant fungi in the botttom piles.The relative abundance of saprophytic fungi increased gradually during the composting of the cow manure,and the bottom pile was higher than that of the top and middle pile.Redundancy analysis showed that water content and temperature had significant effects on fungal community structure(P<0.05).During the composting process of cow manure,the composition of fungal community was different in temporal and spatial.Most fungal communities in the composting were saprophytic trophic.There was a correlation between the changes of fungal community structure and physicochemical factors.

关 键 词：牛粪堆肥 真菌群落结构 高通量测序 不同时期 空间层次 

分 类 号：S141.4[农业科学—肥料学]