河南省结核分枝杆菌MIRU-VNTR和间隔区寡核苷酸分型分析  

Utility of MIRU-VNTR and spoligotyping for Mycobacterium tuberculosis isolates in Henan Province

作　　者：石洁[1] 郑丹薇 朱岩昆[1] 马晓光[1] 王少华[1] 李辉[1] 张国龙 SHI Jie;ZHENG Danwei;ZHU Yankun;MA Xiaoguang;WANG Shaohua;LI Hui;ZHANG Guolong(Henan Province Center for Disease Control and Prevention,Zhengzhou 450016)

机构地区：[1]河南省疾病预防控制中心,郑州450016

出　　处：《郑州大学学报（医学版）》2019年第3期425-430,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划项目(201702277;2018020516)

摘　　要：目的:调查河南省结核分枝杆菌分子流行病学的结构特征。方法:共纳入河南地区668株结核分枝杆菌,进行间隔区寡核苷酸分型(Spoligotyping)和26位点分枝杆菌散在重复单元-数目可变串联重复序列(MIRU-VNTR)分型分析,评估不同MIRU-VNTR位点组合的分辨效能。结果与结论:北京家族基因型菌株有558(83. 53)株,是河南省最流行的结核分枝杆菌,其中典型北京家族基因型(SIT1)是最具优势的基因型(512株,88. 89%)。26位点MIRU-VNTR可将668株菌分成567种基因型,其中含有38个基因簇和529种独立的基因型。累积Hunter-Gaston分辨力指数表明10个分辨力最强VNTR位点组合的分辨效力接近26位点组合。Aim:To investigate the molecular epidemical population structure of mycobacterium tuberculosis(M.tuberculosis)in Henan.Methods:A total of 668 M.tuberculosis isolates in Henan were analyzed via spoligotyping and 26-locus MIRU-VNTR and different combinations of MIRU-VNTR loci.Results and Conclusion:The Beijing genotype was the most prevalent family(n=558),among which,shared-type(SIT1)was the most dominant genotype(88.89%,n=512).A total of 668 isolates were divided into 567 different types,comprised of 38 clusters(2-15 isolates per cluster)and 529 unique types by 26-locus MIRU-VNTR analysis.An informative set of 10 loci had similar discriminatory power with 26 loci set.

关 键 词：结核分枝杆菌 MIRU-VNTR 间隔区寡核苷酸分型 河南省 

分 类 号：R378.91[医药卫生—病原生物学]