QuEChERS/气相色谱-质谱/质谱法测定蔬菜中58种高风险农药残留量  

Determination of 58 High Risk Pesticide Residues in Vegetables by QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry

作　　者：李凌璞 张建莹 林珊 赵琼晖 钟恬恬 洪旭然 LI Lingpu;ZHANG Jianying;LIN Shan;ZHAO Qionghui;ZHONG Tiantian;HONG Xuran(Food Inspection&Quarantine Center,Shenzhen Customs,Shenzhen 518045,China)

机构地区：[1]深圳海关食品检验检疫技术中心,广东深圳518045

出　　处：《常州大学学报（自然科学版）》2019年第3期40-48,共9页Journal of Changzhou University：Natural Science Edition

基　　金：国家质检总局资助项目(2017IK271;2017IK262)

摘　　要：优化QuEChERS前处理方法,结合气相色谱-串联四极杆质谱技术,建立了蔬菜中58种禁限用和高风险农药残留测定方法。样品用含体积分数为0.1%乙酸的乙腈溶液提取,经基质分散固相萃取净化,气相色谱-质谱/质谱法测定。结果表明:在线性范围0.010～0.200 mg/L内,58种农药的线性相关系数(r^2)均大于0.99,定量限均为0.010 mg/kg。分析物添加水平在0.010,0.050 mg/kg和0.100 mg/kg时,回收率范围在71.2%～120%,相对标准偏差(RSD)为1.68%～14.8%。方法快速简便、灵敏度高,重现性和稳定性好,适用于日常蔬菜中农药多残留的检测。A method for the determination of 58 banned,restricted and high risk pesticide residues in vegetables was developed by gas chromatography-tandem quadrupole mass spectrometry(GC-MS/MS) with modified QuEChERS pretreatment method.The sample was extracted with acetonitrile containing 0.1% acetic acid,cleaned-up with dispersive solid phase extraction,determined by GC-MS/MS.The results showed that the calibration curves of 58 pesticides were linear in the range of 0.010-0.200 mg/L with correlation coefficients ( r2 ) higher than 0.99.The limits of quantitations for the method were 0.010 mg/kg.The recoveries were 71.2%-120% with added concentration as 0.010,0.050 mg/kg and 0.100 mg/kg,the RSD of the method were between 1.68% and 14.8%.The method showed the advantages of quickness,simplicity,high sensitivity,good reproducibility and stability,and was suitable for the analysis of pesticide residues in daily vegetables.

关 键 词：QUECHERS 气相色谱-质谱/质谱 农药残留 

分 类 号：O652.1[理学—分析化学]