一种示踪干细胞的磁共振-荧光双模成像探针（英文）  

A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking

作　　者：赵敏敏 张艳辉[2] 张朋利[2] 谭波[2] 蒋海珍[1] 张海禄[2,3,4,5] 邓宗武 ZHAO Min-min;ZHANG Yan-hui;ZHANG Peng-li;TAN Bo;JIANG Hai-zhen;ZHANG Hai-lu;DENG Zong-wu(College of Sciences,Shanghai University,Shanghai 200444,China;Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences,Suzhou 215123,China)

机构地区：[1]上海大学理学院,上海200444 [2]中国科学院苏州纳米技术与纳米仿生研究所,江苏苏州215123 [3]江苏省核磁共振专业委员会 [4]中华放射学会磁共振物理与工程学组 [5]中国晶体学会药物学专业委员会

出　　处：《波谱学杂志》2019年第2期127-137,共11页Chinese Journal of Magnetic Resonance

基　　金：the National Natural Science Foundation of China(31371010,21673281);Youth Innovation Promotion Association of the Chinese Academy of Sciences(2012242);Key Research Project from the Ministry of Science and Technology of China(2017YFA0104300)

摘　　要：本文设计并合成了Gd基磁共振-荧光双模成像探针——Gd-DOTA-PEG-GA,通过电穿孔的方式标记人源间充质干细胞(hMSCs).电穿孔标记诱导细胞将探针组装成团簇状纳米粒子进入细胞质,显著延长其与细胞结合的时间,并呈现出明显的T2信号减弱效应,且信号减弱效应可以持续7天以上.在水溶液中,该探针的发射带集中在498nm,并且荧光强度在一周内无明显衰减.该探针标记的细胞在荧光倒置显微镜下呈现绿色荧光.这些结果表明该探针可以作为磁共振-荧光双模成像探针用于干细胞示踪.A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self -assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T2-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo.

关 键 词：磁共振成像 荧光成像 双模探针 人源间充质干细胞 

分 类 号：O482.53[理学—固体物理]