雌、孕激素对Meis1在人子宫内膜细胞中的表达调控  

Expression and regulation of MEIS1 in human endometrial cells by estrogen and progesterone stimuli

作　　者：李海霞[1] 夏正明[2] 钱坤[3] 章汉旺[3] 胡琳莉[4] 夏薇[1] LI Haixia;XIA Zhengming;QIAN Kun;ZHANG Hanwang;HU Linli;XIA Wei(Medical Reproduction Center,Medical Reproduction Center,Guangzhou First People s Hospital,Guangzhou 510180,China;The Second Affiliated Hospital of Guangzhou Meidcal University,Guangzhou 510000,China;Medical Reproduction Center,Department of Obstetrics & Gynecology,TongJi Hospital,TongJi Medical College,Huazhong University of Science & Technology,Wuhan 430030,China;Medical Reproduction Center,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]广州市第一人民医院生殖医学中心,广州510180 [2]广州医科大学附属第二医院西院区,广州510000 [3]华中科技大学同济医学院附属同济医院妇产科生殖医学中心,武汉430030 [4]郑州大学第一附属医院生殖医学中心,郑州450000

出　　处：《广州医药》2019年第3期6-10,共5页Guangzhou Medical Journal

基　　金：国家自然科学基金(30700909);广东省中医药局课题(20141045)

摘　　要：目的探讨Meis1在人子宫内膜细胞中的表达及其受雌、孕激素调控的规律。方法通过免疫细胞化学和western blot的方法检测雌、孕激素对体外培养的在子宫内膜基质细胞(ESC)及Ishikawa细胞中Meis1的表达及调控。结果 Meis1在ESC和Ishikawa细胞均有表达,且均表达于细胞核中;在ESC中,E2、P4和E2+P4三组中Meis1平均蛋白表达水平均高于对照组(P<0.05)。Meis1在E2、P4和E2+P4组之间的表达水平亦差异有统计学意义(P<0.05),表达强度E2+P4组>P4组>E2组;在Ishikawa细胞中,E2、P4和E2+P4使Meis1表达增强,表达强度P4组>E2+P4组>E2组,但与对照组比较无差异(P>0.05),E2、P4和E2+P4各组间亦无差异(P>0.05)。结论转录因子Meis1在ESC和Ishikawa细胞中受到雌、孕激素的调控,可能在子宫内膜容受性分子网络的构建中发挥着重要的作用。Objective To investegate the expression and regulation discipline of Meis1 in human ESC and Ishikawa cells in vitro by estrogen and progesterone stimuli. Methods Immunocytochemistry and western blot were used to detect the expression and regulation discipline of Meis1 in human normal endometrial cells. Results Meis1 expressed both in endometrial stromal cells (ESC) and in ishikawa cells,and both situ in nucleus. In ESC,the expression of Meis1 was up-regulated by E 2/P 4 and E 2+P 4,and the up-regulated effect may be superposition by E 2+P 4,the differences between the groups were statistically difference( P <0.05). In Ishikawa cells,western blot showed that the expression of Meis1 was up-regulated by E 2/P 4 and E 2+P 4. The differences weren t statistically significant when compared with the control group or between themselves( P >0.05). Conclusion The expression of transcription factor Meis1 is regularly regulated by estrogen and progesterone,which may be a key role during the formation of endometrial receptivity molecular network.

关 键 词：Meis1 ESC ISHIKAWA细胞 子宫内膜容受性 

分 类 号：R714.8[医药卫生—妇产科学]