唑拉西泮和替来他明对中华草龟的麻醉效果及其药物代谢动力学  

Anaesthetic effects of zolazepam and tiletamine on Chinemys reevesiis and pharmacokinetics of zolazepam and tiletamine

作　　者：马艳 潘连德[1] 徐建平 郑串 MA Yan;PAN Liande;XU Jianping;ZHENG Chuan(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;College of Food Sciences and Technology,Quality and Safety Risk Assessment Laboratory of Aquatic Products Storage,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学上海省部共建水产种质资源发掘与利用教育部重点实验室水产科学国家级实验教学示范中心农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学食品学院农业农村部水产品贮藏保鲜质量安全风险评估实验室,上海201306

出　　处：《水产学报》2019年第5期1397-1406,共10页Journal of Fisheries of China

基　　金：水产动物遗传育种上海市协同创新中心(ZF1206)~~

摘　　要：为了解龟类临床兽医学的麻醉术问题,根据预实验结果选择动物医学推荐的临床麻醉药物舒泰(唑拉西泮:替来他明=1∶1)对中华草龟进行麻醉实验,比较不同注射剂量的舒泰对中华草龟的麻醉效果,并使用高效液相色谱法(HPLC)测定舒泰中唑拉西泮和替来他明在中华草龟血浆中的浓度,用药物代谢动力学软件PKsolver 2.0来分析其药物代谢动力学特征。结果发现,肌肉注射舒泰剂量为25 mg/kg时,对中华草龟麻醉的诱导期、麻醉期和苏醒期的时长分别为0.42、4.33和4.14 h,舒泰对中华草龟的麻醉效果较好,符合临床手术的要求。唑拉西泮和替来他明在中华草龟体内的药时曲线均符合一次式二室开放模型,唑拉西泮的药物代谢动力学方程为C=13.462e^(–0.223t)+2.127e^(–0.055t),主要药物代谢动力学参数为达峰浓度C_(max)=11.695μg/mL、达峰时间T_(max)=1.060 h和药时曲线下面积AUC=92.470μg/(mL·h);替来他明的药物代谢动力学方程为C=10.619e–0.203t+0.279e–0.047t,主要药物代谢动力学参数为达峰浓度Cmax=9.654μg/mL、达峰时间Tmax=0.482 h和药时曲线下面积AUC=56.348μg/(mL·h)。结合麻醉效果分析,唑拉西泮和替来他明血药浓度分别达到8.12和8.96μg/mL时,可以使健康的中华草龟进入麻醉期,为维持麻醉状态,唑拉西泮和替来他明的血药浓度应不低于7.18和4.73μg/mL。The current study aimed to solve the problems of anaesthesia in clinical veterinary medicine of turtles. According to the results of pre-experiment, we selected the animal medicine recommended clinical anaesthetic drugs Telazol(tiletamine:zolazepam=1:1). We compared the anaesthetic effects of different dosages of Zoletil on Chinemys reevesiis, and the concentrations of zolazepam and tiletamine in plasma were detected by High Performance Liquid Chromatogram (HPLC), and analyzed with software PKsolver 2.0 to research the features of pharmacokinetics. Zoletil at a rate of 25 mg/kg was administered intramuscularly to C. reevesiis.The induction time, anaesthesia time and recovery time were 0.42, 4.33 and 4.14 h respectively. Zoletil on C. reevesiis anaesthesia was better, in line with the requirements of clinical surgery. The concentration-time curves of zolazepam and tiletamine in plasma all were conformed to a two-compartment open model. And the concentration- time equations of zolazepam and tiletamine were C=13.462e^-0.223t+2.127e^-0.055t, C=10.619e^-0.203t+0.279e^-0.047t, respectively. The major pharmacokinetic parameters were as follows: Cmax=11.695 μg/mL, Tmax=1.060 h and AUC=92.470 μg/(mL h) in zolazepam;Cmax=9.654 μg/mL, Tmax=0.482 h and AUC=56.348 μg/(mL h) in tiletamine. It suggested that blood concentration of zolazepam and tiletamine all reached 8.12 μg/mL and 8.96 μg/ mL, respectively, which could cause healthy C. reevesiis into anaesthesia. To maintain anaesthesia, the blood concentrations of zolazepam and tiletamine should not be less than 7.18 μg/mL and 4.73 μg/mL.

关 键 词：中华草龟 唑拉西泮 替来他明 麻醉 药物代谢动力学 

分 类 号：S948[农业科学—水产养殖]