云南西盟蔗区发现检疫性病害甘蔗白叶病  

A Quarantining Sugarcane White Leaf Found in Cane-growing Regions in Ximeng, Yunnan Province

作　　者：张荣跃[1] 李文凤[1] 黄应昆 王晓燕[1] 单红丽[1] 李婕 仓晓燕[1] 罗志明[1] 尹炯[1] Zhang Rongyue;Li Wenfeng;Huang Yingkun;Wang Xiaoyan;Shan Hongli;Li Jie;Cang Xiaoyan;Luo Zhiming;Yin Jiong(Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan ProvinceKey Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, Yunnan, China)

机构地区：[1]云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远661699

出　　处：《农学学报》2019年第5期20-23,共4页Journal of Agriculture

基　　金：国家自然科学基金项目"甘蔗白叶病植原体的传播媒介及其传毒机理研究"(31760504);云南省农业基础研究联合专项"云南甘蔗白叶病植原体分子流行学研究"[2017FG001(-054)];国家现代农业产业技术体系(糖料)建设专项资金"甘蔗真菌病害防控"(CARS-170303);云岭产业技术领军人才培养项目"甘蔗有害生物防控"(2018LJRC56);云南省现代农业产业技术体系建设专项资金"植物保护与病害研究"(YNGZTX-4-92)

摘　　要：研究旨在明确2018年在云南西盟蔗区发现的甘蔗病害是否为植原体引起的甘蔗白叶病。使用植原体16S rRNA基因序列通用引物P1/P7和R16F2n/R16R2对10份采自云南西盟蔗区的疑似甘蔗白叶病样品进行巢氏PCR检测,并将巢氏PCR产物进行克隆测序和序列分析。巢氏PCR结果表明:所有10份样品均能扩增得到1240 bp左右的片段。测序结果表明所有获得的10条16S rRNA基因序列大小均为1247 bp,序列间的核苷酸一致性为100%。BLASTN分析表明,从病株扩增到的所有核苷酸序列与先前云南临沧甘蔗白叶病植原体分离物LC7和LC9的16S rRNA基因序列(Genbank登陆号:KR020691和KR020692)的核苷酸序列一致性为100%。虚拟RFLP分析表明,西盟分离物的16S r RNA基因序列的酶切图谱与16SrXI-B亚组植原体相同。本研究结果表明,云南西盟蔗区发现的甘蔗病害为16SrXI-B亚组植原体引起的甘蔗白叶病。The study investigated whether the sugarcane disease found in cane-growing regions in Ximeng,Yunnan Province in 2018 was sugarcane white leaf caused by phytoplasma. We performed nested PCR with phytoplasma universal primer pairs(P1/P7 and R16 F2 n/R16 R2) to identify 16 S rRNA genes for detection of SCWL phytoplasma in 10 suspected SCWL samples collected from Ximeng. Amplified products were cloned,sequenced and analyzed. The results of nested PCR showed that about 1240 bp nested PCR amplified fragment could be obtained from all 10 samples. The sequencing results showed that the 10 16 S rRNA gene sequences were all 1247 bp in length and the nucleotide identity between the sequences was 100%. BLASTN analysis revealed that all nucleotide sequences amplified from the diseased plants shared 100% sequence similarity with the nucleotide sequence of 16 S rRNA genes from SCWL phytoplasma isolates LC7 and LC9 from Lincang,Yunnan Province(Genbank accession no. KR020691 and KR020692). The virtual RFLP analysis indicated that the virtual RFLP patterns of the 16 S rRNA gene sequence of the Ximeng isolates were identical with those of subgroup 16 SrXI-B. Thus, we confirm that sugarcane disease in Ximeng, Yunnan Province is SCWL caused by subgroup 16 SrXI-B phytoplasma.

关 键 词：云南 西盟 检疫性病害 植原体 甘蔗白叶病 16SrXI-B亚组 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]