鼠李糖乳杆菌GG株对铅中毒子代鼠的保护作用  

Protective effect of Lactobacillus rhamnosus GG on lead-poisoning offspring mice

作　　者：冷星宇 俞勤斐 周凡 沈薇[3] 孟晓静[1] 吴娴波[1] 覃旻[1] 范宏英[1] LENG Xingyu;YU Qinfei;ZHOU Fan;SHEN Wei;MENG Xiaojing;WU Xianbo;QIN Min;FAN Hongying(School of Public Health,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学公共卫生学院,广州510515 [2]毕节市第三人民医院,贵州毕节551500 [3]南方医科大学南方医院新生儿科,广州510515

出　　处：《实用医学杂志》2019年第10期1555-1559,1565,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:31872630;81773473);广东大学生科技创新培育专项资金项目(攀登计划专项)(编号:PDJH2017b0102);广东省科技计划项目(编号:2016A020217010);广东省医学科研基金项目(编号:B2017051)

摘　　要：目的探讨鼠李糖乳杆菌GG株(LGG)对母鼠暴露铅致子代鼠铅中毒的保护作用。方法将24只6周龄无特定病原体(SPF)级C57BL/6鼠,按雌雄比1:1随机分为4组:空白对照(NC)组、铅暴露(Pb)组、益生菌干预(LGG.Pb)组及益生菌(LGG)组,Pb组和LGG.Pb组每日自由饮用200 mg/L无菌醋酸铅水,其他组饮用无菌去离子水。LGG.Pb组和LGG组每日灌胃109CFU的LGG活菌,其他组灌胃等容量无菌磷酸缓冲盐溶液,直至子代鼠21日龄处死,取血、骨、脑、肾、肝脏及小肠,进行铅浓度检测,检测谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、一氧化氮(NO)等氧化应激因子,并对大脑及小肠作苏木素伊红(HE)染色,评价其病理损伤情况。结果与NC组相比,Pb组子代鼠血、骨、脑和肾组织的铅浓度升高,肝脏中GSH、GSH-Px、SOD等活性降低,NO活性升高,差异均有统计学意义(P <0.05);脑及小肠出现病理损伤。与Pb组相比,LGG.Pb组子代鼠血铅、脑铅、骨铅和肾铅浓度降低,肝脏SOD、GSH、GSH-Px活性升高,而NO活性降低,差异均有统计学意义(P <0.05);脑及小肠的病理损伤得到改善。结论 LGG对母鼠铅暴露致铅中毒子代鼠具有保护作用。Objective To investigate the protective effect of Lactobacillus rhamnosus GG(LGG)on leadpoisoning offspring mice induced by maternal lead exposure. Methods Twenty-four 6-week-old specific pathogen free C57 BL/6 mice were randomly divided into 4 groups by the gender ration of 1∶1:no-treatment control(NC)group,lead exposure(Pb)group,probiotics-intervention(LGG.Pb)group and probiotics(LGG)group. Pb and LGG.Pb group received 200 mg/L sterile lead acetate water for free drink while others sterile deionized water. 109 CFU LGG viable bacteria were administered to LGG.Pb and LGG group daily,while aseptic phosphate buffer saline of equal volume were administered to other groups then 21-day-old offspringmice were sacrificed. Blood,bone,brain and kidney were collected for detecting lead concentration;liver for measuring the activity of glutathione(GSH),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and nitric oxide(NO)and brain and small intestine biopsies were visualized by hematoxylin-eosin staining. Results Compared with those in the NC group,lead concentration of blood,brain,bone and kidney was increased;activity of GSH,GSH-Px and SOD in liver was decreased while that of NO was increased,and pathological lesions were found in brain and small intestine in the Pb group. However,compared with those in the Pb group,the lead concentration of those tissues/organs was decreased(P < 0.05)while the activity of GSH,GSH-Px and SOD was increased(P < 0.05)and that of NO was decreased(P < 0.05)in liver of the LGG.Pb group. Meanwhile,the pathological lesions were markedly improved.Conclusion LGG can protect the lead-poisoning offspring mice induced by maternal lead exposure.

关 键 词：鼠李糖乳杆菌GG株 铅中毒 母婴传播 氧化应激 病理损伤 

分 类 号：R595[医药卫生—内科学]