橡胶树茉莉酸信号途径相关基因表达与橡胶产量的相关性  

Correlation between expression level of genes related to jasmonate signalling and rubber yield

作　　者：杨署光[1] 赵悦 陈月异[1] 李言[1] 张世鑫[1] 田维敏[1] YANG Shuguang;ZHAO Yue;CHEN Yueyi;LI Yan;ZHANG Shixin;TIAN Weimin(Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Rubber Biology and Genetic Resources of RubberTree, Minstry of Agriculture and Rural Affoirs, P. R. China/State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops,Danzhou 571737, Hainan, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 10093, China)

机构地区：[1]中国热带农业科学院橡胶研究所农业农村部橡胶树生物学与遗传资源利用重点实验室省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737 [2]中国农业大学农学院,北京100193

出　　处：《广西植物》2019年第5期641-649,共9页Guihaia

基　　金：国家青年科学基金(31800577);国家天然橡胶产业技术体系分子育种岗位专项项目(CARS-34-GW1);海南省自然科学基金(20153136);中国热带农业科学院橡胶研究所基本科研业务费专项项目(1630022016003;1630022017027)~~

摘　　要：割胶促进橡胶树合成天然橡胶与激活乳管细胞的茉莉酸信号途径密切相关,但茉莉酸信号途径关键环节的基因表达水平与干胶产量的相关性尚不清楚。为了找到与产量相关的分子标记,该研究采用qPCR技术,分析了割胶条件下茉莉酸信号途径关键环节的9个相关基因在5个橡胶树魏克汉种质和5个1981’IRRDB种质乳管细胞中的表达。结果表明:大多魏克汉种质的株次干胶产量显著高于1981’IRRDB种质。在9个基因中,除了HbMYC4和HbMYC5,其余7个基因在大多橡胶树魏克汉种质中的表达量均显著高于1981’IRRDB种质,尤其是HbMYC3基因表达差异性好,与干胶产量相关性高,有望作为橡胶树产量育种的一个分子标记。这对育种周期长的橡胶树产量育种具有实际应用价值。Tapping-enhanced rubber biosynthesis is closely related to the activation of jasmonate signaling in laticifer cells of rubber tree. The relationship between the expression level of the genes involved in jasmonate signaling and dry rubber yield remains not elucidated. In the present study, the expression of nine genes related to jasmonate signaling was analyzed by qPCR in the laticifer cells of five Wichham germplasms and 5 1981’IRRDB germplasms upon tapping with S/2 d/3 tapping system. The rubber yield per tapping of most Wichham germplasms was significantly higher than that of 1981’IRRDB germplasms. Except for HbMYC4 and HbMYC5 , the expression level of the other seven genes in most of Wichham germplasms was significantly higher than that of 1981’IRRDB germplasms. It was noted that the expression of HbMYC3 was highly different and closely related to the rubber yield, which may be used as a candidate marker for rubber yield-breeding of rubber tree.

关 键 词：巴西橡胶树 割胶 橡胶生物合成 茉莉酸信号途径 HbCOI1 HbJAZs HbMYCs 基因表达 

分 类 号：Q943.2[生物学—植物学]