血培养病原菌分布及厌氧瓶的临床应用价值研究  

The Research on Pathogens Isolated from Blood Culture and the Clinical Application of Anaerobic Culture Vials

作　　者：段宁 杨江辉[2] 王利君[1] 肖楠[1] 祝天阳 DUAN Ning;YANG Jiang-hui;WANG Li-jun;XIAO Nan;ZHU Tian-yang(Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University,Beijing 102218,China;Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University,Beijing 102218,China)

机构地区：[1]清华大学附属北京清华长庚医院,清华大学临床医学院检验科,北京102218 [2]清华大学附属北京清华长庚医院,清华大学临床医学院病理科,北京102218

出　　处：《标记免疫分析与临床》2019年第5期826-829,共4页Labeled Immunoassays and Clinical Medicine

基　　金：北京市医院管理局“青苗”计划专项(编号:QML20152203)

摘　　要：目的研究血培养瓶中病原菌分布及血培养阳性报警时间(TTP),探讨厌氧瓶在血培养中的临床价值。方法分析2016年1月至2017年7月3406套血培养数据,Wilcoxon符号秩检验成套血培养中需氧瓶和厌氧瓶TTP差异。结果血培养分离573株菌,其中大肠埃希菌166株(29.0%)、凝固酶阴性葡萄球菌76株(13.3%)、肺炎克雷伯菌72株(12.6%)、肠球菌55株(9.6%)、金黄色葡萄球菌27株(4.7%),且这些菌在厌氧瓶阳性而需氧瓶阴性的比例分别是22.3%、23.5%、26.1%、18.2%、5.9%。血培养厌氧瓶TTP明显短于需氧瓶(P<0.001)。结论厌氧瓶能提高常见兼性厌氧菌的检出率并缩短检出时间,其临床应用价值大。Objective The aim of the study is to evaluate the clinical significance of anaerobic culture vials through analyzing the pathogenic distribution and the time-to-positivity(TTP)of blood culture vials.Methods A total of 3,406 sets of blood culture were collected from Jan,2016 to Jul,2017.The value of TTP was analyzed by Wilcoxon signed-rank test.Results A total of 573 isolates were identified,including:166(29%)Escherichia coli,76(13.3%)Coagulase negative staphylococcus,72(12.6%)Klebsiella pneumonia,55( 9.6%)Enterococcus.spp,and 27(4.7%)Staphylococcus aureus,while accounted for 22.3%,23.5%,26.1%,18.2%,and 5.9% of above pathogens,which were only isolated in anaerobic vials in the absence of any aerobic vial.TTP of anaerobic vials was shorter than that of aerobic vials(P< 0.001 ).Conclusion The anaerobic vial can promote the positive rate and turn-around time of the most common facultative anaerobes,suggesting a clinical practice value.

关 键 词：血培养 阳性报警时间 厌氧瓶 

分 类 号：R446.5[医药卫生—诊断学]