我国猪群中多杀性巴氏杆菌的基因型分析  

Genotypical Characteristics of Swine Pasteurella multocida in China

作　　者：彭忠[1] 梁婉[1,2] 艾伟诚 王斐 华琳 汤细彪 陈焕春[1] 吴斌[1] PENG Zhong;LIANG Wan;AI Weicheng;WANG Fei;HUA Lin;TANG Xibiao;CHEN Huanchun;WU Bin(State Key Laboratory of Agricultural Microbiology,The Cooperative Innovation Center for Sustainable Pig Production,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture),Institute of Animal Husbandry and Veterinary Science,Hubei Academy of Agricultural Sciences,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院农业微生物学国家重点实验室生猪健康养殖协同创新中心,武汉430070 [2]湖北省农业科学院畜牧兽医研究所农业部畜禽细菌病防治制剂创制重点实验室,武汉430070

出　　处：《畜牧兽医学报》2019年第5期1064-1072,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：湖北省农业科技创新行动项目(2018skjcx05);国家科技支撑计划项目(2015BAD12B04)

摘　　要：本研究旨在了解我国猪群中流行的多杀性巴氏杆菌(Pm)的主要基因型。利用荚膜基因分型、脂多糖(LPS)基因分型、多位点序列分型(MLST)对2013年12月—2017年12月4年间来源于我国各地区规模化猪场患有疑似呼吸系统疾病的病死猪肺、鼻拭子、气管、肝等样品中分离鉴定的Pm进行基因型分析,并对23种主要毒力基因进行检测。结果表明,当前在我国猪群中流行的Pm的荚膜基因型为A(48.85%)、D(42.75%)、F(2.67%),优势基因型为A和D;LPS基因型为L3(25.00%)和L6(75.00%)型,优势基因型为L6;MLST基因型为ST3(21.25%)、ST10(27.50%)、ST11(42.50%)、ST12(2.50%)、ST16(2.50%)、ST74(1.25%)及ST75(2.50%),优势基因型为ST3、ST10和ST11。如果将荚膜基因型、LPS基因型和MLST基因型组合起来看,当前在我国猪群中流行的Pm的主要基因型为荚膜:脂多糖:MLST基因型A:L3:ST3(20.00%)、A:L6:ST10(26.25%)及D:L6:ST11(42.50%)。毒力基因检测的结果发现部分毒力基因的分布表现出一定的"基因型偏好性"。结果提示,D:L6:ST11是我国猪群中流行的多杀性巴氏杆菌的主要基因型,可能与我国猪群中由多杀性巴氏杆菌导致的呼吸道疾病密切相关。The aim of this study was to understand the genotypical characteristics of swine P.mul- tocida currently circulating in China.Capsular genotyping,LPS genotyping,MLST genotyping were performed on P.multocida isolates from the clinical samples including lungs,nasal swabs,tracheas,livers,and other tissues of pigs with respiratory disorders in different farms in different regions of China's Mainland;virulence genotyping based on the detection of different virulence gene profiles was also set for the isolates recovered herein.The results showed that the capsular genotypes of the swine P.multocida currently circulating in China were A (48.85%),D (42.75%),and F (2.67%);the LPS genotypes were L3 (25.00%) and L6 (75.00%);the MLST genotypes were ST3 (21.25%),ST10 (27.50%),ST11 (42.50%),ST12 (2.50%),ST16 (2.50%),ST74 (1.25%),and ST75 (2.50%).When combining the capsular genotypes with the LPS genotypes and the MLST genotypes,it was found that the predominant capsule:LPS:MLST genotypes prevalent in pigs in China were A:L3:ST3 (20.00%),A:L6:ST10 (26.25%),and D:L6:ST11 (42.50%).During the virulence genotyping,some virulence-associated genes were found to show a certain level of “genotype-preference”,which means the detection rate of those genes in some genotypes is significantly higher than their detection in other genotypes.These results indicate that a capsule:LPS:MLST genotype D:L6:ST11 of P.multocida is likely to be strongly associated with swine respiratory disease in China.

关 键 词：猪多杀性巴氏杆菌 荚膜基因型 脂多糖基因型 MLST基因型 毒力基因 

分 类 号：S852.612[农业科学—基础兽医学]