4种地被植物干旱胁迫下的生理响应及抗旱性评价  

Responses of four ground cover plants to drought stress and the comprehensive evaluation on drought resistance capacity

作　　者：李艳[1,2] 王庆 刘国宇[1,2] 张燕 杨群力[1,2] 杜勇军 李仁娜[1,2] 王玮 LI Yan;WANG Qing;LIU Guoyu;ZHANG Yan;YANG Qunli;DU Yongjun;LI Renna;WANG Wei(Xi'an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi'an 710061, Shaanxi, China;Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi'an 710061, Shaanxi, China)

机构地区：[1]陕西省西安植物园陕西省植物研究所,陕西西安710061 [2]陕西省植物资源保护与利用工程技术研究中心,陕西西安710061

出　　处：《中南林业科技大学学报》2019年第6期9-15,共7页Journal of Central South University of Forestry & Technology

基　　金：陕西省科学院重大科研专项(2017K-15);陕西省科学院优秀青年科研暨青年联合创新项目(2017K-04);陕西省重点研发计划(2017NY-024)(2017SF-307)

摘　　要：为了丰富关中地区园林地被植物种类,让园林企业在合理选择和应用地被植物有可靠的理论依据,以关中地区引种栽培的顶花板凳果、大吴风草、迷迭香和蜘蛛抱蛋4种常绿地被植物2年生盆栽苗为试材,采用人工控水模拟水分胁迫的方法,研究其在不同程度下的植株形态变化,并测定4种植物的萎蔫系数及叶片的游离脯氨酸、MDA、SOD、POD、CAT、可溶性蛋白以及可溶性糖等生理生化指标的变化,由此来综合评价其抗旱性。结果表明:1)从干旱临界值来看,迷迭香的最低,达到3.01%;2)从叶片游离脯氨酸含量变化来看,顶花板凳果、大吴风草及迷迭香随着干旱胁迫程度的增加呈现增长趋势,到了重度干旱胁迫,其含量分别达到对照的7.09倍、11.73倍、18.59倍;3)从MDA含量变化来看,大吴风草和迷迭香叶片中的含量同干旱胁迫程度呈正相关,蜘蛛抱蛋、顶花板凳果则整体呈现缓慢的下降趋势;4)随着干旱胁迫程度的增加,4种植物叶片可溶性蛋白含量变化均有所增加,但差异不显著。可溶性糖的含量只有蜘蛛抱蛋呈现先升后降的趋势,其余3种均呈现显著连续下降趋势;5)从叶片保护酶活性分析,干旱胁迫条件下SOD、POD和CAT均有所增加。其中顶花板凳果、大吴风草及蜘蛛抱蛋的CAT含量显著增加,增长率分别达159.28%、239.31%和200.97%;6)利用隶属函数分析4种常绿地被植物的抗旱性强弱,其顺序为:迷迭香>顶花板凳果>蜘蛛抱蛋>大吴风草,这一结果与盆栽干旱试验的形态表现一致。In order to enrich the species of groundcover plants in guanzhong area, and the garden enterprises have a reliable theoretical basis for the reasonable selection and application of groundcover plants,the potted two years old plantlets of four evergreen ground cover plants were used as the test materials in this study. Four introduction and cultivation plants, Pachysandra terminalis, Farfugium japonicum, Rosmarinus officinalis and Aspidistra elatior were examined to study their drought resistance by using artificial water control simulated water stress method. Some physiological and morphological indices, such as wilting coefficient, free proline content, MDA, SOD, POD, CAT, soluble protein, soluble sugar of leaves were investigated and analysed after drought stress treatment. Drought resistance of four evergreen ground cover plants were comprehensively evaluated. The result show that: 1) In terms of drought threshold, Rosmarinus officinalis was the lowest, reaching 3.01%;2) from the change of free proline content in leaves, Pachysandra terminalis, Farfugium japonicum and Rosmarinus officinalis increased with the increase of the degree of drought stress, respectively 7.09 times, 11.73 times and 18.59 times of the control;3) from the MDA content changes, the content of the leaves of Farfugium japonicum, Rosmarinus officinalis was positively correlated with the degree of drought stress, and the content of the leaves of Aspidistra elatior and Pachysandra terminalis showed a slow downward trend;4) with the increase of drought stress, the content of soluble protein in the leaves of four plants increased, but the difference was not significant.The content of soluble sugar of Aspidistra elatior showed a trend of rising first and then falling , while the other three presented a significant continuous downward trend;5) SOD, POD and CAT were increased under drought stress.The content of CAT of Pachysandra terminalis, Farfugium japonicum, and Aspidistra elatior was significantly increased, the rate of increase were 159.28%,

关 键 词：地被植物 抗旱性 干旱胁迫 综合评价 隶属函数法 

分 类 号：S718.5[农业科学—林学]