异养鞭毛虫对铜绿微囊藻的生长影响  

Effects of Heterotrophic Flagellates (Paraphysomonas sp.) on Growth Performance of Microcystis aeruginosa

作　　者：罗晓霞[1,2,3] 李长玲 何雪怡[1] 陶德琴 杜振雄 LUO Xiao-xia;LI Chang-ling;HE Xue-yi;TAO De-qin;DU Zhen-xiong(College of Fisheries, Guangdong Ocean University, Zhanjiang 524088,China;Engineering Technology Research Center for Algae Breeding and Application of Guangdong Province, Zhanjiang 524088,China;Shenzhen Research Institute of Guangdong Ocean University, Shenzhen 518000,China)

机构地区：[1]广东海洋大学水产学院 [2]广东省藻类养殖及应用工程技术研究中心,广东湛江524088 [3]广东海洋大学深圳研究院,广东深圳518000

出　　处：《广东海洋大学学报》2019年第3期38-45,共8页Journal of Guangdong Ocean University

基　　金：广东省自然科学基金项目(2018A030313212);广东海洋大学自然科学研究项目(C17378);广东海洋大学博士启动项目(R17039);大学生创新创业训练计划项目(CXXL2019043)

摘　　要：【目的】研究异养鞭毛虫(Paraphysomonas sp.)在不同密度铜绿微囊藻(Microcystis aeruginosa)中的生长状况,探讨其对铜绿微囊藻的摄食及抑制作用。【方法】实验共设6个铜绿微囊藻密度,分别是0、250×10~4、500×10~4、750×10~4、1 000×10~4和2 000×10~4cells/mL,研究异养鞭毛虫与微囊藻的种群数量变化。【结果】异养鞭毛虫主要行异养生活。在铜绿微囊藻密度为250×10~4~2 000×10~4 cells/mL时,异养鞭毛虫对铜绿微囊藻的抑制率为98.00%~99.69%。单个鞭毛虫对微囊藻的摄食率为13~49 cells/d。异养鞭毛虫的密度和生长率随铜绿微囊藻密度的升高而增加,在铜绿微囊藻密度为500×10~4~1000×10~4cells/mL时,异养鞭毛虫密度为21×10~4~24×10~4cells/mL、生长率为0.6~0.74 d^(-1)。但是,铜绿微囊藻密度过高也不利于异养鞭毛虫的种群增长,在2 000×10~4 cells/mL密度时,异养鞭毛虫的生长速率显著低于其它密度组。【结论】异养鞭毛虫能有效抑制铜绿微囊藻种群增长,其生长的最适微囊藻密度为500×10~4~1 000×10~4 cells/mL。【Objective】The growth status of heterotrophic flagellates Paraphysomonas sp. in different concentrations of Microcystis aeruginosa and its inhibitory effect on M. aeruginosa were studied.【Method】M. aeruginosa was designated with six different concentrations, which were 0, 250×10^4,500×10^4, 750×10^4, 1 000×10^4 and 2 000×10^4 cells·mL^-1, respectively.【Result】Paraphysomonas sp. is mainly heterotrophic life and it could inhibit the growth of M. aeruginosa with concentration ranged from 250×10^4 to 2 000 ×10^4 cells·mL^-1. The inhibition rate was 98 to 99.7%, and the ingestion rate of single flagellate was 13-49 cells·day^-1. The number and growth rate of heterotrophic flagellates increased with the increased concentration of M. aeruginosa and reached the highest peak at the concentration of 500×10^4-1 000×10^4 cells·mL^-1. The number and growth rate of heterotrophic flagellates reached 21×10^4-24×10^4 cells/mL, 0.6-0.74 d^-1 respectively. However, the excessive concentration of M. aeruginosa did not favor the population growth of heterotrophic flagellates, and the growth rate of heterotrophic flagellates in the 2 000×104 cells·ml^-1 treatment was significantly lower than that of other concentration treatments.【Conclusion】The flagellates can effectively inhibit the growth of M. aeruginosa, and the optimal M. aeruginosa concentration for the growth of heterotrophic flagellates is 500×10^4 to 1 000×10^4 cells·mL^-1.

关 键 词：铜绿微囊藻 异养鞭毛虫 生长率 抑制率 摄食率 

分 类 号：Q954.4[生物学—动物学]