鹿角DNA提取方法研究  

The Research of Methods for DNA Extraction in Antler

作　　者：王磊[1] 张然然[1] 王洪亮[1] 李洋 胡鹏飞[1] 帕尔哈提 邢秀梅[1] WANG Lei;ZHANG Ran-ran;WANG Hong-liang;LI Yang;HU Peng-fei;Paerhati;XING Xiu-mei(State Key Laboratory of Special Economic Animal Molecular Biology,Insitute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所特种经济动物分子生物学重点实验室,长春130112

出　　处：《特产研究》2019年第2期38-41,共4页Special Wild Economic Animal and Plant Research

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-2017-ISAPS)

摘　　要：收集63支鹿角,利用酚-氯仿法、改良酚-氯仿法、DNeasy~? Blood&Tissue Kit (50)、QIAamp~? DNA Investigator Kit (50)提取鹿角DNA,通过观察琼脂糖凝胶电泳结果,确定提取鹿角DNA的取样部位和提取方法。结果表明,鹿角中部DNA含量高于基部,更适合用于鹿角DNA提取工作;酚-氯仿法优于改良酚-氯仿法,DNeasy~? Blood&Tissue Kit (50)提取效果优于其他方法。进行鹿角DNA提取时,建议在鹿角中部取样,用DNeasy~? Blood&Tissue Kit (50)提取DNA。The methods〔Phenol-chloroform method, Modified phenol-chloroform method, DNeasy■ Blood & Tissue Kit(50) method,QIAamp■ DNA Investigator Kit(50) method〕were respectively used to extract DNA from 63 antlers. By observing the results of agarose gel electrophoresis, the sampling site and extraction method for antler DNA extraction were determined. The results showed that the DNA content in the middle of antler was higher than the base, which was more suitable for DNA extraction of antler. The phenol-chloroform method was superior to the modified phenol-chloroform method, and the DNeasy~? Blood & Tissue Kit(50) was better than other methods. For antler DNA extraction, it was recommended to take samples in the middle of the antler and extract DNA using the DNeasy~? Blood & Tissue Kit(50).

关 键 词：鹿角 DNA提取 琼脂糖凝胶电泳 

分 类 号：Q781[生物学—分子生物学]