母鼠成年前至孕哺期硒与甲基汞联合暴露对子代大鼠小脑的损伤  

Antagonism of selenium to cerebellar injury in offspring induced by meternal exposure to methylmercury from immature period to pregnancy and lactation

作　　者：涂瑞 丁丽[2] 陈敏 张婵婵 冯琳 王惠群 李平 TU Rui;DING Li;CHEN Min;ZHANG Chan-chan;FENG Lin;WANG Hui-qun;LI Ping(Department of Nutrition and Food Hygiene,School of Public Health,Guizhou Medical Universily,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州医科大学公共卫生学院营养与食品卫生学教研室,贵州贵阳550025 [2]贵州医科大学环境与疾病监控教育部重点实验室 [3]中国科学院地球化学研究所环境地球化学国家重点实验室

出　　处：《环境与健康杂志》2018年第9期777-780,F0003,共5页Journal of Environment and Health

基　　金：国家自然科学基金(41373135;41622208)

摘　　要：目的初步探讨硒(selenium,Se)对甲基汞(methylmercury,MeHg)染毒仔鼠小脑损伤作用的影响。方法将24只清洁级雌性SD大鼠按体重随机分为4组,分别为对照组(生理盐水)、硒(0.2 mg/kg)染毒组、甲基汞(1.2 mg/kg)染毒组和甲基汞+硒染毒组,每组6只。采用经口灌胃方式染毒,从交配前8周、妊娠期和哺乳期(出生21 d)连续给母鼠染毒。断乳后,测定仔鼠脑汞、硒的水平,检测小脑组织丙二醛(malondialdehyde,MDA)含量和硫氧还蛋白氧化还原酶(thioredoxin reductase,TrxR)活力。结果甲基汞染毒组和甲基汞+硒染毒组仔鼠脑汞含量高于对照组和硒染毒组,差异均有统计学意义(P<0.05);与甲基汞染毒组相比,甲基汞+硒染毒组仔鼠脑汞含量较高,差异有统计学意义(P<0.05)。硒染毒组和甲基汞+硒染毒组仔鼠脑硒含量高于对照组和甲基汞染毒组,差异均有统计学意义(P<0.05);与硒染毒组相比,甲基汞+硒染毒组仔鼠脑硒含量较高,差异有统计学意义(P<0.05)。与对照组相比,甲基汞染毒组仔鼠小脑MDA含量较高,而TrxR活力较低,差异均有统计学意义(P<0.05);硒染毒组和甲基汞+硒染毒组仔鼠小脑MDA含量和TrxR活力与对照组相比,差异无统计学意义(P>0.05)。结论母鼠成年前至孕哺期甲基汞染毒对子代大鼠小脑造成明显的氧化应激损伤;而硒对甲基汞暴露所致子代大鼠的氧化损伤作用具有拮抗作用。Objective To understand the antagonism of selenium(Se)to cerebellar injury in the offspring rats exposed to methylmercury(MeHg).Methods Twenty-four clean grade female SD rats were randomly divided into four groups by body weight,the control group(normal saline),Se exposure group(0.2 mg/kg),MeHg exposure group(1.2 mg/kg)and MeHg+Se exposure group.The rats were treated with MeHg through gavage eight weeks before mating,during pregnancy and lactation(21 days).The levels of mercury and selenium in the brain of offspring rats were determined.The content of malondialdehyde(MDA)and the activity of thioredoxin reductase(TrxR)in the cerebellum of offspring rats were measured.Results The contents of mercury in the brain were significantly higher in the MeHg exposure group and the MeHg+Se exposure group than that in the control group and the Se exposure group(P<0.05).Compared with the MeHg exposure group,the content of mercury in brain in MeHg+Se exposure group was higher(P<0.05).The contents of selenium in the brain of offspring were significantly higher in the Se exposure group and the MeHg+Se exposure group than that in the control group and the MeHg exposure group(P<0.05).Compared with the Se exposure group,the content of selenium in brain in MeHg+Se exposure group was higher(P<0.05).The MDA content was higher and the TrxR activity was lower in MeHg exposure group compared with the control group(P<0.05);There was no significant difference in MDA content and TrxR activity between Se exposed group and MeHg+Se group compared with control group(P>0.05).Conclusion Meternal exposure to methylmercury from immature period to pregnancy and lactation can cause obvious oxidative stress damage in the cerebellum of the offspring rats,which can be antagonized by selenium.

关 键 词：甲基汞 硒 联合染毒 小脑损伤 

分 类 号：R994.6[医药卫生—毒理学]