毛蕊花糖苷对过氧化氢诱导脑胶质细胞氧化损伤的保护作用  

Protective effect of verbascoside on brain glial cells with oxidative stress injury

作　　者：李朝晖[1] 权威[2] 石天济 谭诚 时景伟[3] Li Zhaohui;Quan Wei;Shi Tianji;Tan Cheng;Shi Jingwei(Department of Neurosurgery,China-Japan Union Hospital of Jilin University,Changchun 130033,China;Department of Neurology,China-Japan Union Hospital of Jilin University,Changchun 130033,China;Department of Laboratory,China-Japan Union Hospital of Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学中日联谊医院神经外科,长春130033 [2]吉林大学中日联谊医院神经内科,长春130033 [3]吉林大学中日联谊医院检验科,长春130033

出　　处：《中华实验外科杂志》2019年第5期867-869,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察毛蕊花糖苷对氧化应激损伤人脑胶质细胞(HEB)的保护作用。方法体外培养HEB,实验分为模型组、毛蕊花糖苷低、中、高剂量组、阳性药物组及正常对照组。除正常对照组外,其余各组培养液中加入终质量浓度为100 mmol/L的过氧化氢(H2O2)制备HEB氧化应激损伤,其中毛蕊花糖苷低、中、高剂量组分别加入终质量浓度为20、40、80μg/L的毛蕊花糖苷;阳性药物组加入终质量浓度为40μg/L的维生素C;正常对照组加入等体积的完全培养基。共同培养4 h后,光镜观察HEB形态变化;噻唑蓝(MTT)法测定细胞存活率;酶联免疫吸附试验(ELISA)法测定培养液中乳酸脱氢酶(LDH)浓度,分光光度法测定HEB中超氧化物歧化酶(SOD)活性和丙二醛(MDA)浓度。应用SPSS 17.0统计软件进行分析。结果与正常对照组比较,模型组HEB皱缩,伪足回缩,体积变小,细胞培养液中悬浮细胞较多。与模型组比较,毛蕊花糖苷各浓度组及阳性药物组HEB伪足回缩少,细胞数量明显增多。模型组细胞存活率为(38±6)%,毛蕊花糖苷低、中、高浓度组细胞存活率分别为(45±2)%、(63±4)%、(83±7)%,阳性药物组细胞存活率为(83±2)%。与模型组比较,毛蕊花糖苷各浓度组及阳性药物组细胞存活率明显增高(t=3.120、3.920、3.892,P<0.01)。正常对照组SOD水平为1.83±0.64,MDA水平为6.25±0.47,细胞培养液中LDH浓度为1 357.78±110.24;模型组HEB细胞中SOD水平为0.94±0.21,MDA水平为14.35±1.17,细胞培养液中LDH浓度为1 994.32±88.25。与正常对照组比较,模型组HEB细胞SOD水平降低(t=4.289,P<0.01),MDA水平升高(t=3.204,P<0.01),细胞培养液中LDH浓度升高(t=3.056,P<0.01)。毛蕊花糖苷中、高剂量组和阳性药物组细胞SOD水平分别为1.56±0.41、1.86±0.74、1.78±0.47,MDA浓度分别为8.06±0.43、6.84±0.91、6.63±0.25,细胞培养液中LDH浓度分别为1 548.36±109.47、1 384.39±107.95、1 297.81.36±114.32。与模型组比较,�Objective To investigate the protective effect of verbascoside on brain glial cells(HEB)with oxidative stress injured.Methods HEB cells were cultured by dulbeeeo modified eagle medium(DMEM)culture liquid containing 10%fetal bovine serum and randomly divided in to model group,verbascoside low,medium and high dose group,positive drug group and normal control group.Except normal control group,the other group were induced to oxidative stress injury by bing exposed to H2O2 at dose 100 mmol/L.20,40,80μg/L verbascoside were separately added into low,medium and high doses of verbascoside groups.Afer co-culturing for 4 h,the morphological changes of HEB were observed by light microscope;the survival rate of HEB was examined using methyl thiazol tetrazolium(MTT).The activity of superoxide dismutase(SOD)and the level of malondialdehyde(MDA)in the HEB were detected with spectrophotometry.The levels of lactate dehydrogenase(LDH)in culture medium were determined by enzyme linked immunosorbent assay(ELISA).Statisticalanalysis of data using the statistical product and service solutions 17.0 software.Results Compared with the normal control group,HEB in model group shrank,more suspension cells were in the culture medium,Compared with the model group,HEB cells in each concentration group and positive drug group retracted less and the number of cells increased significantly.The cell survival rate of model group was(38±6)%.The cell survival rates of low,medium and high concentration of pistil glycoside were(45±2)%,(63±4)%and(83±7)%,respectively.The cell survival rate of positive drug group was(83±2)%.Compared with the model group,the cell viability of the three concentration groups and the positive drug group increased significantly(t=3.120,3.920,0.892,P<0.01).In the normal control group,SOD level was 1.83±0.64,MDA level was 6.25±0.47 and LDH concentration in cell culture medium was 1357.78±110.24.In model group,SOD level was 0.94±0.21,MDA level was 14.35±1.17 and LDH concentration in cell culture medium was 1 994.32±88.

关 键 词：毛蕊花糖苷 氧化应激 脑胶质细胞 

分 类 号：R285.5[医药卫生—中药学]