丁苯酞在脑损伤后炎性反应中的神经保护机制  

The role and mechanism of dl-3-n-butylphthalide in inflammatory response after traumatic brain injury in mice

作　　者：刘政委[1] 李立文 王汉东[2] 石小峰[1] 孔文龙[1] Liu Zhengwei;Li Liwen;Wang Handong;Shi Xiaofeng;Kong Wenlong(Department of Neurosurgery,Shenzhen Longgang Central Hospital,the Ninth People’s Hospital of Shenzhen City,Shenzhen 518116,China;Department of Neurosurgery,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,China)

机构地区：[1]深圳市龙岗中心医院、深圳市第九人民医院神经外科,518116 [2]南京军区南京总医院神经外科,210002

出　　处：《中华实验外科杂志》2019年第5期877-879,共3页Chinese Journal of Experimental Surgery

基　　金：深圳市知识创新计划项目(20170227164955321).

摘　　要：目的通过小鼠颅脑创伤模型,探讨丁苯酞是否通过抑制创伤后小胶质细胞活化进而抑制炎性反应,进而可以发挥保护神经细胞的作用。方法144只实验小鼠随机均分为4组,分别为:假手术组(Sham)、外伤组[脑损伤(TBI)]、外伤+溶剂组(TBI+V)和外伤+丁苯酞(NBP)组(TBI+NBP),每组36只。对每组小鼠进行神经功能评分,脑组织切片尼氏(Nissl)染色;对小鼠脑组织进行免疫荧光染色定位小胶质细胞蛋白抗体(IBA-1)在小鼠皮层脑组织的分布和使用酶联免疫吸附法(ELISA)检测白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)在小鼠挫裂伤脑组织的含量。应用SPSS 22.0统计软件进行分析。结果丁苯酞改善颅脑损伤后小鼠的神经功能评分,缓解脑创伤后的神经元变性(t=6.320、5.440,P<0.05)。丁苯酞可以抑制小鼠脑组织创伤后IBA-1蛋白的表达,抑制炎性因子TNF-α[(7.21±1.21)pg/mg]和IL-1β[(3.52±1.13)pg/mg,t=3.370、3.420、3.350、3.400,P<0.05]。结论小鼠脑损伤后使用丁苯酞药物可以抑制小胶质细胞的活化而起到了神经保护作用,其保护作用与减少促炎细胞因子的释放相关。Objective To investigate whether butylphthalide(NBP)can protect neurons by inhibiting microglial activation and inflammation after traumatic brain injury in mice.Methods One hundred and forty-four mice were randomly assigned to four experimental groups:sham,traumatic brain injury(TBI),TBI+vehicle(V)and TBI+NBP.Neurological score,Nissl staining were performed in each group of mice,immunofluorescence staining was used to localize the distribution of ionized calcium binding adapter molecule 1(IBA-1)in the cerebral cortex of mice,and enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the contusion and laceration brain tissue of mice.Statisticalanalysis of data using the statistical product and service solutions 22.0 software.Results NBP could improve the neurological function score,relieve neurodegeneration after brain injury in mice(t=6.320,5.440,P<0.05).NBP can inhibit the expression of IBA-1 protein and the inflammatory factors TNF-α[(7.21±1.21)pg/mg]and IL-1β[(3.52±1.13)pg/mg]after brain injury in mice(t=3.370,3.420,3.350,3.400,P<0.05).Conclusion NBP can inhibit the activation of microglia in mice after TBI,which is related to the decrease of the release of pro-inflammatory cytokines.

关 键 词：创伤性颅脑损伤 丁苯酞 神经保护 炎性因子 

分 类 号：R651.15[医药卫生—外科学] R-332[医药卫生—临床医学]